Biolegend, 931402, HA Peptide Tag (Previously Covance catalog# PEP-101P), 5mg

Influenza hemagglutinin (HA) is a viral membrane glycoprotein responsible for binding of the virus to the cells expressing sialic acid-containing surface receptors, such as erythrocytes and the cells in the upper respiratory tract. The hemagglutinin epitope, YPYDVPDYA, has been widely used as a tag in recombinant protein expression vectors.
5mg
Purity: >90% pure by HPLC
Formulation: Lyophilized powder.
Preparation: Lyophilized (trace TFA salts).
Storage & Handling: Store at -20°C or below. Do not store in frost-free freezers.
Application: ELISA, IP, WB
Recommended Usage: Each lot of this product is quality control tested by ELISA. Soluble in PBS. The optimal working dilution should be determined for each specific assay condition. • Elution of epitope tag fusion proteins from immunoaffinity columns: 400 ug/mL • Competitive inhibition of antibody in western or immunostaining: 10-fold molar excess over optimized (low) antibody
Application Notes: This synthetic peptide (H2N-CYPYDVPDYA-COOH) corresponds to the epitope detected by the anti-HA HA.11 monoclonal antibody. This peptide can be used in competition experiments, or to elute bound HA-fusion proteins from the HA.11 affinity matrix (Cat. No. 900801).
Application References(PubMed link indicates BioLegend citation): Kizhatil K, et al. 2002. J. Neurosci. 22: 7948. Ferrando A, et al. 2001. Nucleic Acids Res. 29:3685.
Product Citations: Amer-Sarsour F, et al. 2024. EMBO J. 250:43. PubMed
Biology Area: Cell Biology
Gene ID: NA
UniProt: View information about HA Tag on UniProt.org
Regulatory Status: RUO
Other Names: HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag
Previously: Covance Catalog# PEP-101P