Biolegend, 932002, Purified anti-E1 Ubiquitin Activating Enzyme Antibody, 100μg

The proteasomal pathway of protein degradation involves two discrete steps: ubiquitination and degradation. Aside from proteasomal degradation, ubiquitination also plays an important role in transcriptional regulation, protein trafficking including endocytosis and lysosomal targeting, and the activation of kinases involved in signaling processes. Ubiquitination is carried out in three main steps: activation, conjugation, and ligation. E1 Ubiquitin Activating Enzyme (UBE1) initiates the cascade of conjugation by activating their respective modifiers. It is highly conserved in human, yeast, and plants. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Mixture of human and mouse E1 protein.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2.0 µg per ml. For immunocytochemistry, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Salvat C, et al. 2000. Eur. J. Biochem. 267:3712.
RRID: AB_2565603 (BioLegend Cat. No. 932002)
Structure: 1058 amino acids with a predicted molecular weight of approximately 118 kD.
Distribution: Ubiquitous, Eukaryotic cells.
Function: This protein catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation.
Interaction: GAN.
Biology Area: Cell Biology, DNA Repair/Replication, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance, Signal Transduction, Transcription Factors, Ubiquitin/Protein Degradation
Molecular Family: Protein Modifiers
Antigen References: 1. Xu W, et al. 2013. Curr. Pharm. Des. 18:3201. 2. Neutzner M, et al. 2012. Essays Biochem. 52:37. 3. Schulman BA, et al. 2009. Nat. Rev. Mol. Cell Biol. 10:319. 4. Salvat C, et al. 2000. Eur. J. Biochem. 267:3712.
Gene ID: 7317
UniProt: View information about E1 Ubiquitin Activating Enzyme on UniProt.org
Clone: 2G2.3.5
Regulatory Status: RUO
Other Names: Ubiquitin-Activating Enzyme E1, Ubiquitin-Like Modifier Activating Enzyme 1, UBE1, A1S9T, GXP1, SMAX2, 2G.2.3.5, 2G2.3-5
Isotype: Mouse IgG1, κ