Biolegend, 932302, Purified anti-Hepatitis B Surface Antigen (HBsAg) Antibody, 100μg

Hepatitis B is a liver infection caused by the Hepatitis B virus (HBV). Hepatitis B surface antigen (HBsAg) contains the large (L), middle (M), and small (S) surface proteins of HBV. HBsAg can be detected at high levels in serum during acute or chronic HBV infection. The body normally produces antibodies to HBsAg as part of the normal immune response to infection. HBsAg is the antigen used to make the HBV vaccine and is also a diagnostic marker.
100μg
Verified Reactivity: Hepatatis B virus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified HBsAg from human HBsAg-positive serum.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested WB - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Huang T, et al. 2005. J. Biol. Chem. 280:27742. (WB) Wang H, et al. 2003. Am. J. Pathol. 163:2441. (WB) Shih H, et al. 2008. J. Virol. 82:2250. (WB) Shih H, et al. 2012. J. Virol. 86:9044. (WB) Hsu S, et al. 2004. J. Virol. 78:6. (WB)
Product Citations: Giadans CG, et al. 2019. J Viral Hepat. 26:727. PubMed Biswas S, et al. 2022. Nat Commun. 13:2995. PubMed
RRID: AB_2566428 (BioLegend Cat. No. 932302)
Structure: Hepatitis B surface antigen (HBsAg) contains the large (L), middle (M), and small (S) surface proteins of Hepatitis B virus (HBV).
Distribution: HBsAg is on the surface of HBV that is present in bodily fluids and hepatocytes of infected individuals.
Function: HBsAg plays a key role in HBV's infection of hepatocytic cells.
Interaction: Hepatocytes.
Ligand/Receptor: Several hepatocytic receptors including asialoglycoprotein receptors and Annexin V.
Biology Area: Cell Biology
Antigen References: 1. Toita B, et al. 2015. World J. Gastroenterol. 24:7400-7411.
Gene ID: 944569
UniProt: View information about HBsAg on UniProt.org
Clone: A10F1
Regulatory Status: RUO
Other Names: HBsAg, Major surface protein, Large S protein, Hepatitis B Surface Antigen, ayw subtype, Australian antigen
Isotype: Mouse IgG2b, κ