Biolegend, 933001, Purified anti-53BP1 Antibody, 25μg

p53-binding protein 1 (53BP1) was first discovered as an interacting partner of p53 tumor suppressor protein in a yeast two-hybrid screening. 53BP1 binds to p53 through a BRCA1 C-terminal (BRCT) domain and enhances p53-dependent transcriptional activation. 53BP1 has been studied extensively for its function in the regulation of DNA double-strand break (DSB) repair pathway. Additionally, it promotes non-homologous end joining (NHEJ) while inhibiting homologous recombination (HR) by preventing DNA end resection. Upon DSB, the ubiquitin ligase RNF168 ubiquitylates lysine 15 on histone H2A/H2AX, which serves as a binding site for 53BP1. 53BP1 is then phosphorylated by ATM, which facilitates the recruitment of downstream effector proteins, including PTIP and RIF1. 53BP1-mediated NHEJ is essential for V(D)J recombination and class switch recombination.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human 53BP1 protein.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone recognizes both human and mouse 53BP1.This clone was tested for ICC using both Triton X-100 and methanol permeabilization with both methods showing correct staining.This clone failed IP testing.Predicted MW of 53BP1 is 214 kD but the observed MW is ~350kD. An identical observed MW was noted when comparing to other control antibodies on the market. Similar differences in predicted and observed molecular weights are reported with other antibodies on the market.
Product Citations: Zhang L, et al. 2022. Nat Commun. 13:360. PubMed
RRID: AB_2820201 (BioLegend Cat. No. 933001) AB_2820202 (BioLegend Cat. No. 933002)
Structure: 53BP1 is a 1972 amino acid protein with a predicted molecular weight of 214 kD.
Distribution: Nucleus/Ubiquitously expressed
Function: DNA damage response
Biology Area: DNA Repair/Replication
Antigen References: Iwabuchi K, et al. 1994. Proc Natl Acad Sci USA. 91:6098. Wang B, et al. 2002. Science. 298:1435. Difilippantonio S, et al. 2008. Nature. 456:529. Bunting SF, et al. 2010. Cell. 141:243. Cuella-Martin R, et al. 2016. Molecular Cell. 64:51.
Gene ID: 7158
UniProt: View information about 53BP1 on UniProt.org
Clone: W17184B
Regulatory Status: RUO
Other Names: Tumor Protein P53 Binding Protein 1, TP53-Binding Protein 1, P53-Binding Protein 1, P53BP1, Tumor Suppressor P53-Binding Protein 1, TDRD30, P202
Isotype: Rat IgG2a, κ