Biolegend, 933203, Ultra-LEAF™ Purified anti-human CCL8 (MPC-2) Antibody, 100μg

CCL8, also known as MCP-2, is a beta chemokine initially isolated from human MG-63 osteosarcoma cells stimulated with IL-1β. CCL8 (MCP-2) has structural and functional similarity with MCP-1 and MCP-3, and it is often co-induced with those chemokines in mononuclear cells. IFN-γ is a strong inducer of CCL8 in monocytes and fibroblasts. CCL8 can be processed and secreted as a truncated chemokine CCL8 (6-75). This isoform does not chemoattract monocytes, but it binds to the CCR2 receptor and induces its internalization; as a result, CCL8 inhibits MCP-1 (CCL2) and MCP-2 (CCL8) ERK signaling and antagonizes the chemotactic activity of several CCR2 ligands (MCP-1/CCL2, MCP-2/CCL8, MCP-3/CCL7). CCL8 is highly expressed in the skin, and it serves as an agonist for the chemokine receptor CCR8, but not for CCR2. Therefore, it has been speculated that the CCL8-CCR8 interaction is a crucial regulator of Th2 cell homing, which drives IL-5-mediated chronic allergic inflammation. In addition, CCL8 binds CCR5 receptor with high affinity and inhibits the replication of human immunodeficiency virus type 1. CCL8 is highly expressed in different human diseases, and it is a potential biomarker for the diagnosis of graft-versus-host diseases and tuberculosis.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Human CCL8
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Neut - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by neutralization of the chemotaxis induced by recombinant human CCL8 on human BaF3-hCCR1 transfectants chemoattractant cells. ND50 range: 0.05 - 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2861115 (BioLegend Cat. No. 933203) AB_2861116 (BioLegend Cat. No. 933204)
Structure: Chemokine
Distribution: Fibroblast, endothelial cells, monocytes, and macrophages
Function: CCL8 chemoattracts monocytes, T cells, NK cells, mast cells, eosinophils, and basophils. CCL8 is induced by IL-1β, IFNβ, INFγ, dsRNA, virus, ConA, and anti-CD2. The induction of CCL8 by IFNγ synergizes with the TLR ligands peptidoglycan (TLR2), dsRNA (TLR3), and LPS (TLR4).
Interaction: Monocytes, T cells, NK cells, mast cells, eosinophils, and basophils
Ligand/Receptor: CCR1 (CD191), CCR2 (CD192), CCR3 (CD193), and CCR5 (CD195)
Cell Type: Basophils, Eosinophils, Mast cells, Monocytes, NK cells, T cells
Biology Area: Cell Biology, Neuroinflammation, Neuroscience, Signal Transduction
Molecular Family: Cytokines/Chemokines
Antigen References: Van Damme J, et al. 1992. J Exp Med. 176:59-65. Proost P, et al. 1998. J Immunol. 160:4034-41. Dean RA, et al. 2008. Blood. 112: 3455-64. Hori T, et al. 2008. Blood. 111:4403-12. Rom S, et al. 2010. FASEB J. 24:2292-300. Struyf S, et al. 2009. Eur J Immunol. 39:843-57. Islam SA, et al. 2011. Nat Immunol. 12:167-77.
Gene ID: 6355
UniProt: View information about CCL8 on UniProt.org
Clone: A15150G
Regulatory Status: RUO
Other Names: Monocyte chemotactic protein 2 (MCP-2), C-C Motif Chemokine Ligand 8
Isotype: Mouse IgG2a, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.