Biolegend, 933902, Purified anti-EGFR Antibody, 100μg

Epidermal Growth Factor Receptor (EGFR) is a receptor tyrosine kinase that links extracellular mitogenic ligand binding to complex downstream signaling cascades. Initial ligand binding results in receptor oligomerization and autophosphorylation of multiple tyrosine residues within cytosolic domains of the the protein. These phosphorylation events stabilize the EGFR kinase activation loop and lead to the recruitment of adaptor proteins and other downstream effectors. EGFR stimulation leads to activation of RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCγ-PKC and STAT signaling cascades, and constitutive activation of the receptor promotes tumorigenesis in multiple cancers.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide from human EGFR
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IHC-P, ICFC, IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.5 - 5 µg/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone was tested for ICC using 4% PFA-fixed A431 cells permabilized with methanol or Triton X-100. While both permeabilization methods were compatible with the antibody, methanol enabled superior staining. This clone is predicted to recognize isoform I based off of complete sequence homology of the immunizing peptide and the corresponding region of the isoform.
Product Citations: He J, et al. 2023. J Extracell Vesicles. e12364:12. PubMed Huang CC, et al. 2020. Biosens Bioelectron. 169:112362. PubMed
RRID: AB_2820215 (BioLegend Cat. No. 933901) AB_2820216 (BioLegend Cat. No. 933902)
Structure: EGFR is a 1,210 amino acid protein with a predicted molecular weight of 134 kD; the processed form is 1,186 amino acids in length.
Distribution: Plasma membrane/Ubiquitously expressed
Function: EGFR signaling, cell signaling
Cell Type: Endothelial cells, Epithelial cells
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family: CD Molecules, Growth Factors
Antigen References: Hunter T and Cooper JA. 1981. Cell. 24:741. Gill GN and Lazar CS. 1981. Nature. 293:305. Reynolds FH, et al. 1981. Nature. 292:259 Zhou M, et al. 2013. Cancer Res. 23:7056-67
Gene ID: 1956
UniProt: View information about EGFR on UniProt.org
Clone: A19002A
Regulatory Status: RUO
Other Names: Epidermal Growth Factor Receptor, Receptor Tyrosine-Protein Kinase ErbB-1, Erb-B2 Receptor Tyrosine Kinase 1, ERBB1, NISBD2, PIG61, MENA, Proto-oncogene c-ErbB-1
Isotype: Mouse IgG1, κ