Biolegend, 934202, Purified anti-PPM1B Antibody, 100μg

Protein phosphatase 1B (PPM1B) is a member of the Protein Phosphatase 2C (PP2C) family of monomeric metal-dependent Ser/Thr protein phosphatases which are dependent on Mg2+ and Mn2+ for their catalytic activity, and function in the negative regulation of cellular stress signaling pathways. PPM1B contributes to the regulation of both cell senescence and proliferation as a regulator of the p38 MAPK pathway and negatively regulates necroptosis through dephosphorylation of receptor-interacting protein 2 (RIP3), one of the primary mediators of necrotic programmed cell death. It is one of a limited number of proteins that can dephosphorylate nuclear receptor PPARγ, thereby stimulating its transcription and contributing to its role in cellular differentiation and metabolism. It is also associated with the dephosphorylation of p53, TAK1, and, in some cell types, the negative regulation of NF-κB signaling through dephosphorylation of nuclear factor kappa B kinase subunit β (IKKβ). PPM1B plays a role in the mediation of antiviral response by acting as a TBK1 phosphatase via dephosphorylation at Ser172 and suppresses cancer cell proliferation and tumor progression through the dephosphorylation of Rho guanine nucleotide dissociation inhibitor 1 (RhoGDI1), a key regulator of Rho GTPases.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human PPM1B protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedKO/KD-WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone failed to stain PPM1B in HeLa cells fixed with 4% PFA and permeabilized with either Triton X-100 or methanol. It also failed to stain cells fixed and permeabilized with methanol.
Additional Product Notes: This clone failed to stain PPM1B in HeLa cells fixed with 4% PFA and permeabilized with either Triton X-100 or methanol. It also failed to stain cells fixed and permeabilized with methanol.
RRID: AB_2894521 (BioLegend Cat. No. 934201) AB_2894521 (BioLegend Cat. No. 934202)
Structure: PPM1B is 479 amino acid protein with a predicted molecular weight of 52 kD.
Distribution: Ubiquitously expressed/ cytosol and membrane
Function: Protein phosphatase
Biology Area: Cell Biology
Molecular Family: Protein Kinases/Phosphatase
Antigen References: Cho H, et al. 2018. Cancer Letters. 417:141 Park J, et al. 2014. Mech Ageing Dev. 138:45. Tasdelen I, et al. 2013. Biochem J. 451:45. Wanz C, et al2015. Nat Cell Biol. 17:434. Zhao Y, et al. 2012. Cell Signal. 24:2197.
Gene ID: 5495
UniProt: View information about PPM1B on UniProt.org
Clone: W19264A
Regulatory Status: RUO
Other Names: Protein Phosphatase 1B, PP2CB, PP2C-beta, PP2C-beta-X, Protein Phosphatase, Mg2+/Mn2+ Dependent 1B, Ser/Thr Protein Phosphatase Type 2C Beta 2 Isoform
Isotype: Rat IgG2b, κ