Biolegend, 934801, Purified anti-IL-18 Antibody, 25μg

Interleukin 18 (IL-18) is a proinflammatory cytokine that participates in both innate and acquired immunity. IL-18 is a part of the IL-1 superfamily that activates T-helper 1 (Th1) and natural killer (NK) cells by inducing IFN-γ production. IL-18 is produced in its inactive precursor form, pro-IL-18 (24 kD), with no signal peptide, accumulating in the cytoplasm. Pro-IL-18 is then processed intracellularly by CASP1 into the mature active form of IL-18 (18 kD) which is secreted extracellularly. Mature IL-18 binds to an inducible receptor, IL-18Rα, and then recruits IL-18Rβ which initiates an immune reaction through TLR/IL-1R-like signaling by recruiting MyD88 and the NF-κB pathway. IL-18 is associated with Adult-onset Still's disease and pleural tuberculosis.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human IL-18 protein of approximately 60 amino acids
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone recognizes human IL-18. For ICC testing, HeLa cells were fixed in 4% PFA and permeabilized with either Triton X-100 or methanol; both methods produced strong staining at 1 mg/mL.
RRID: AB_2820219 (BioLegend Cat. No. 934801) AB_2820220 (BioLegend Cat. No. 934802)
Structure: IL-18 is a 193 amino acid protein with a predicted molecular weight of 22 kD.
Distribution: IL-18 is secreted, also localized to the nucleus and cytosol.
Function: Proinflammatory cytokine
Ligand/Receptor: Binds inducible receptor IL-18Rα, and then recruits IL-18Rβ
Cell Type: NK cells, T cells
Biology Area: Adaptive Immunity, Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: Adachi O, et al. 1998. Cell Press. 9:1:143. Akira S, et al. 2000. Curr. Op. Imm. 12:1:59. Van de Veerdonk, et al. 2011. Trends in Imm. 32:3:110. Canna S, et al. 2017. Jour. of All. and Clin. Imm. 139:5:1698. Muhl H, et al. 2019. Cell Sig. 63:109388.
Gene ID: 3606
UniProt: View information about IL-18 on UniProt.org
Clone: W18160A
Regulatory Status: RUO
Other Names: IL18, IL-18, Interleukin 18, Interleukin-18, IL-1 Gamma, Interleukin-1 Gamma, IFN-Gamma-Inducing Factor
Isotype: Rat IgG2a, λ