Biolegend, 935401, Purified anti-TIGAR Antibody, 25μg

Tp53-induced glycolysis and apoptosis regulator (TIGAR) is a negative regulator of glycolysis upregulated by p53 in response to cellular stresses and it protects cells against reactive oxygen species (ROS)-induced autophagy. TIGAR decreases the rate of glycolysis by reducing the level of fructose-2, 6-bisphosphate, which is an activator of phosphofructokinase-1 (PFK1), a rate-limiting enzyme in glycolysis. Additionally, TIGAR promotes the pentose phosphate pathway and provides NADPH for glutathione reductase to generate reduced glutathione (GSH). GSH lowers the intracellular level of ROS and prevents DNA damage, thereby promoting cell survival.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human TIGAR protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone does not recognize mouse TIGAR.The clone produced incorrect staining in ICC testing.The clone could not pull down TIGAR in IP testing.
RRID: AB_2832894 (BioLegend Cat. No. 935401) AB_2832895 (BioLegend Cat. No. 935402)
Structure: TIGAR is a 270 amino acid protein with a predicted molecular weight of 30 kD.
Function: Oxidative stress, cancer metabolism
Biology Area: Cell Biology
Molecular Family: Enzymes and Regulators
Antigen References: Green D, et al. 2006. Cell. 126:30. Bensaad K, et al. 2006. Cell. 126:107. Cheung E, et al. 2012. Proc Natl Acad Sci USA. 109:20491. Wanka C, et al. 2012. J. Biol. Chem. 287:33436.
Gene ID: 57103
UniProt: View information about TIGAR on UniProt.org
Clone: W18015A
Regulatory Status: RUO
Other Names: TP53 Induced Glycolysis Regulatory Phosphatase, TP53-Induced Glycolysis And Apoptosis Regulator, Fructose-2,6-Bisphosphatase TIGAR, C12orf5, Fructose-2,6-Bisphosphate 2-Phosphatase, Chromosome 12 Open Reading Frame 5, Transactivated By NS3TP2 Protein
Isotype: Rat IgG2a, κ