Biolegend, 935602, Purified anti-GIT1 Antibody, 100μg

GIT1 is a G protein-coupled receptor kinase-interacting protein which is a component of the PIX/Rac/PAK pathway that is involved in cellular development. In this pathway, Rac activates PAK which leads to the phosphorylation of PIX and GIT1. The downstream result to these interactions leads to the formation of focal adhesion complexes in the periphery of the cell membrane with various proteins including FAK and paxillin. GIT1's various protein-protein interactions in the focal complex lead to downstream cellular events such as migration, morphogenesis and neuronal growth (spine/synapse formation).
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human GIT1 protein of approximately 250 amino acids surrounding amino acid 500
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC, IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Non-specific bands present in HeLa lysates.Triton X-100 is the best ICC permeabilization method for this antibody.
RRID: AB_2832898 (BioLegend Cat. No. 935601) AB_2832899 (BioLegend Cat. No. 935602)
Structure: GIT1 is a 761 amino acid protein with a predicted molecular weight of 84 kD.
Distribution: Cytosol and focal adhesion sites
Function: GTPase activation
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Protein Trafficking and Clearance
Molecular Family: GTPases, Scaffold Proteins
Antigen References: Zhang H, et al. 2005. J. Neurosci. 25:3379. Zhao ZS, et al. 2000. Mol. Cell. Biol. 20:6354. Goehler H, et al. 2004. Mol. Cell. 15:853.
Gene ID: 28964
UniProt: View information about GIT1 on UniProt.org
Clone: W18253B
Regulatory Status: RUO
Other Names: G Protein-Coupled Receptor Kinase Interacting, ArfGAP 1, GRK-Interacting Protein 1, ARF GAP GIT1, CAT1, Cool-associated and tyrosine phosphorylated protein 1, ARF GTPase-activating protein GIT1
Isotype: Rat IgG2a, κ