Biolegend, 937301, Purified anti-ERp57 Antibody, 25μg

ERp57 is a member of the protein disulfide family of endoplasmic reticulum localized oxidoreductases and plays an important role in disulfide bond formation and protein folding. Along with calnexin and calreticulin, it is essential for the synthesis and quality control of nascent glycoproteins in the secretory pathway. It is also a core component of the peptide-loading complex that is required for MHC class I presentation. ERp57 is upregulated in multiple neurodegenerative diseases and cancers, and targeted disruption of ERp57 sensitizes cancer cells to chemotherapeutic agents and ionizing radiation.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human ERp57 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone failed IP testing.
Product Citations: Khaleafi R, et al. 2023. Front Immunol. 1231782:14. PubMed Mancino M, et al. 2023. Microbiol Spectr. e0521122:11. PubMed
RRID: AB_2861126 (BioLegend Cat. No. 937301) AB_2861127 (BioLegend Cat. No. 937302)
Structure: ERp57 is a 505 amino acid protein with a predicted molecular weight of 57 kD.
Distribution: Endoplasmic reticulum/Ubiquitously expressed
Function: Molecular chaperone
Biology Area: Cell Biology, Protein Misfolding and Aggregation, Protein Trafficking and Clearance
Molecular Family: Endoplasmic Reticulum Markers, Enzymes and Regulators
Antigen References: Oliver JD, et al. 1997. Science. 275:86-8. Kienast A, et al. 2007. Nat Immunol. 8:864-72. Hussman M, et al. 2015. Oncotarget. 6:39247-61. Blees A, et al. 2017. Nature. 551:525-528.
Gene ID: 2923
UniProt: View information about ERp57 on UniProt.org
Clone: W19080B
Regulatory Status: RUO
Other Names: Protein Disulfide Isomerase Family A Member 3, PDIA3, Endoplasmic Reticulum Resident Protein 57, GRP58, ER Protein 57, GRP57, Epididymis Secretory Protein Li 269, Epididymis Scretory Sperm Binding Protein Li 93n
Isotype: Rat IgG2a, κ