Biolegend, 937402, Purified anti-RRM2 Antibody, 100μg

Ribonucleotide reductase catalyzes the rate-limiting step for de novo deoxyribonucleotide dNTPs synthesis. It is a heterotetramer comprised of two identical large subunits (RRM1) and two identical small subunits (RRM2). The functional ribonucleotide reductase enzyme converts ribonucleotides to dNTPs, which are utilized for DNA synthesis. Ribonucleotide reductase activity requires a high degree of regulation to maintain a proper balance between dNTP availability and DNA synthesis demands. While RRM1 protein levels are generally constitutive, RRM2 expression is cell-cycle regulated at multiple levels. Dysregulation of RRM2 results in dNTP imbalances and increased misincorporation of dNTPs into newly synthesized DNA, and elevated RRM2 levels have been described in multiple forms of cancer. Targeted disruption of RRM2 has been proposed as a mechanism to drive tumors into a senescent state.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Full-length recombinant human RRM2 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone does not recognize mouse RRM2 by western blot.This clone was tested for ICC using HeLa cells fixed with 4% PFA and permeabilized with Triton X-100 or methanol; RRM2 staining failed under both conditions.
RRID: AB_2876752 (BioLegend Cat. No. 937401) AB_2876752 (BioLegend Cat. No. 937402)
Structure: RRM2 is a 389 amino acid protein with a predicted molecular weight of 45 kD.
Distribution: Cytosol/Ubiquitously expressed
Function: DNA synthesis
Biology Area: Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication
Molecular Family: Enzymes and Regulators
Antigen References: Zhang K, et al. 2009. Mol Cancer. 8:11. Zhang YW, et al. 2009. J Biol Chem. 284:18085-95. D’Angiolella V, et al. 2012. Cell. 149:1023-34.
Gene ID: 6241
UniProt: View information about RRM2 on UniProt.org
Clone: W19033A
Regulatory Status: RUO
Other Names: ribonucletide-diphosphate reductase subunit M2, ribonucleotide reductase small subunit, RRM2, RNR2
Isotype: Rat IgG2b, κ