Biolegend, 937502, Purified anti-VASP Antibody, 100μg

Vasodilator-stimulated phosphoprotein (VASP) is part of the Ena-VASP protein family of adaptor proteins which link the cytoskeletal system to signal transduction pathways. The Ena-VASP proteins are involved in cytoskeletal organization, fibroblast migration, platelet activation and axon guidance. VASP interacts with filametous actin formation, so it plays a role in cell adhesion and motility. VASP has three phosphorylation sites, and VASP phosphorylation has been shown to affect actin binding and its effects on actin polymerization. Diseases associated with VASP include thrombosis and clopidogrel resistance.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial recombinant human VASP
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC, IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1 - 2 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone recognizes human VASP.Clone works well in ICC when cells are permeabilized with either Triton X-100 or methanol.VASP antibody detects endogenous levels of total VASP protein. In certain cell lines 2 bands running at 46 kD and 50 kD can be detected, which correspond to the non-phosphorylated and phosphorylated forms of VASP, respectively.
Application References(PubMed link indicates BioLegend citation): Reinhard M, et al. 1992. EMBO J. 11:2063-2070.
RRID: AB_2876753 (BioLegend Cat. No. 937501) AB_2876753 (BioLegend Cat. No. 937502)
Structure: VASP is a 380 amino acid protein with a predicted molecular weight of 39 kD. Western blot shows two bands at 46 and 50 kD corresponding to non-phosphorylated and phosphorylated VAST respectively.
Distribution: VASP is mainly localized to the plasma membrane with additional localization to the focal adhesion sites and cell junctions.
Function: Cell adhesion, cell motility
Interaction: Actin
Ligand/Receptor: Robo receptor and Sertoli-Sertoli Cell Junction Dynamics
Antigen References: Wilton KM and Billadeau. 2018. J Immunol. 10:2899-2909 Harker AJ, et al. 2019. Mol Biol Cell. 7:851-62 Bear JE, et al. 2002. Cell. 109:509. Reinhard M, et al. 1992. EMBO J. 11:2063.
Gene ID: 7408
UniProt: View information about VASP on UniProt.org
Clone: W18344B
Regulatory Status: RUO
Other Names: VASP, Vasodilator Stimulated Phosphoprotein, Vasodilator-Stimulated Phosphoprotein.
Isotype: Mouse IgG2b, κ