Biolegend, 938201, Purified anti-CK1α Antibody, 25μg

Casein Kinase 1 Alpha 1 (CK1α) is a member of the CK1 family of serine/threonine kinases which phosphorylate various regulatory molecules. CK1α is one of seven different non-allelic casein kinase isoforms, each with distinct signaling partners conferred by the sequence deviation in their C-terminal extensions. It plays a critical role in Wnt/β-catenin signaling through phosphorylation of β-catenin at serine 45 by GSK-3, which primes β-catenin for additional phosphorylation events, and is a critical component of the Axin-GSK-3-β-catenin scaffold. Through its phosphorylation of tumor suppressor p53 at serine 20, CK1α plays an important role in cell cycle regulation, DNA repair, apoptosis, and tumor suppression, thus making it of particular interest as a therapeutic target. Studies suggest that CK1α interacts with keratin-associated protein FAM83H to mediate the organization of keratin filaments and cytoskeletal organization.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Full-length recombinant human CK1α protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone was tested for ICC using HeLa cells fixed with 4% paraformaldehyde and permeabilized with either methanol or Triton X-100. Neither method resulted in CK1a staining. This clone was tested for IP using whole cell extracts prepared from HeLa cells. The clone failed to immunoprecipitate CK1a. This clone recognizes multiple isoforms of CK1a.
RRID: AB_2876759 (BioLegend Cat. No. 938201) AB_2876759 (BioLegend Cat. No. 938202)
Structure: CK1α is a 337 amino acid protein with a predicted molecular weight of 39 kD. It also exists as 365 and 325 amino acid isoforms with predicted molecular weights of 42 and 38 kD, respectively.
Distribution: Ubiquitously expressed/ Cytosolic
Function: Serine/threonine protein kinase
Biology Area: Cell Biology
Molecular Family: Protein Kinases/Phosphatase
Antigen References: Jiang S, et al. 2018. Cell Commun. Signal. 16:23. Kuga T, et al. 2013. J. Cell Sci. 126:4721. Macdonald B, et al. 2009. Dev. Cell. 17:9. Schittek B, and Sinnberg T. 2014. Mol. Cancer. 13:231
Gene ID: 1452
UniProt: View information about CK1alpha on UniProt.org
Clone: W18288B
Regulatory Status: RUO
Other Names: CSNK1A1, CK1, CK1a, HLCDGP1, PRO2975, HEL-S-77p, Casein Kinase 1 Alpha 1, Casein Kinase I Isoform Alpha, Epididymis Secretory Sperm Binding Protein Li 77p
Isotype: Rat IgG2a, κ