Biolegend, 940002, Purified anti-Fumarase Antibody, 100μg

Fumarate hydratase, or fumarase, is a Krebs cycle enzyme that catalyzes the conversion of fumarate to L-malate. A cytosolic isoform of Fumarase has been described that is required for arginine metabolism and the urea cycle. Inactivating germline mutations of the FH gene are found in patients with hereditary leiomyomatosis and renal cell cancer syndrome, which predisposes patients to increased risk of developing uterine and cutaneous leipmyomas and renal cell carcinomas. Mechanistically, elevated fumarate functions as an oncometabolite by inhibiting the activity of α-ketoglutarate dependent enzymes, such as hypoxia-inducible factor prolyl hydroxylases (PHDs) and ten-eleven translocation (TET) dioxygenases.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human fumarase protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone was tested for ICC on HeLa cells fixed with 4% PFA and permeabilized with either methanol or Triton X-100. Both methods were compatible fumarase staining. This clone failed to immunoprecipitate fumarase from HeLa extracts. We therefore do not recommend this clone for the application.
RRID: AB_2890856 (BioLegend Cat. No. 940001) AB_2890856 (BioLegend Cat. No. 940002)
Structure: Fumarase is a 510 amino acid protein with a predicted molecular weight of 55 kD.
Distribution: Ubiquitously expressed/Mitochondrial, Cytosolic
Interaction: H2AZ1
Biology Area: Cell Biology, Mitochondrial Function
Molecular Family: Enzymes and Regulators
Antigen References: Laukka T, et al. 2016. J Biol Chem. 291:4256-65. Sciavoceli M, et al. 2016. Nature. 537:544-547.
Gene ID: 2271
UniProt: View information about Fumarase on UniProt.org
Clone: W18319C
Regulatory Status: RUO
Other Names: Fumarate Hydratase, FH
Isotype: Rat IgG2a, κ