Biolegend, 940204, Alexa Fluor® 647 anti-ERG Antibody, 100μg

ERG (ETS-related gene) is a member of the erythroblast transformation-specific (ETS) family of transcription factors. Expressed in the mesoderm and endothelium of embryos, ERG is vital to embryonic cell differentiation as well as regulation of endothelial homeostasis, a role it sustains beyond the embryonic stage. Through transcriptional regulation of VE-cadherin, ICAM-2, and Claudin-5, ERG maintains endothelial junctional integrity and stability and regulation of the endothelial cytoskeleton through its interactions with HDAC6 and RhoJ. ERG is vital to angiogenesis, bone development, platelet adhesion, urogenital tract development, vascular stability, and proper hematopoiesis regulation. It plays a large role in cellular response to vascular inflammation, endothelial cell barrier function, and inhibition of vascular inflammation. These functions are accomplished largely through ERG-mediation of the Wnt/β-catenin signaling pathway by means of transcriptional regulation of FZD4 and VE-cadherin by ERG and subsequent promotion of β-catenin stability. This in turn promotes endothelial cell proliferation. ERG fusion proteins resulting from chromosomal translocations cause ERG to behave as a proto-oncogene, contributing to prostate cancer, megakaryoblastic leukemia, and Ewing’s sarcoma.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human ERG protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: For use of this antibody in Immunocytochemistry (ICC), we recommend fixation with PFA fixation Buffer (Cat. No. 420801) followed by permeabilization with either Triton X-100 (0.5 %) or ice-cold methanol. For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization using True-Nuclear™ Transcription Factor Buffer set (Cat. No. 424401) or True-Phos™ Perm Buffer (Cat. No. 425401).
RRID: AB_3699101 (BioLegend Cat. No. 940203)
Structure: ERG is a 449 amino acid protein with an expected molecular weight of 54 kD.
Distribution: Ubiquitously expressed/ Nucleus
Function: Transcription factor
Cell Type: Endothelial cells
Biology Area: Cell Biology, Ubiquitin/Protein Degradation
Antigen References: 1. Adamo P, et al. 2016. J. Biol. Chem. 35:4013 2. Birdsey G, et al. 2015. Dev. Cell. 32:82 3. Shah A, et al. 2016. Vascul. Pharmacol. 86:3
Gene ID: 2078
UniProt: View information about ERG on UniProt.org
Clone: W19256A
Regulatory Status: RUO
Other Names: ETS-Related Gene, Transcriptional Regulator ERG, ETS Transcription Factor ERG, Erythroblast Transformation-Specific Transcription Factor ERG Variant 10, p55
Isotype: Rat IgG2a, κ