Biolegend, 940402, Purified anti-SARS-CoV-2 S Protein S1 Recombinant Antibody, 100μg

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a single- stranded RNA virus that belongs in a family of viruses known as coronaviruses.   SARS-CoV-2 infection, known as COVID-19, was declared a pandemic by WHO on March 11, 2020 and among other symptoms leads to respiratory infection, pulmonary failure which can be fatal. SARS-CoV-2 is structurally composed of 4 main proteins (e.g. spike glycoprotein, envelope glycoprotein, membrane glycoprotein and nucleocapsid protein) and several accessory proteins. The spike glycoprotein (S) is a transmembrane molecule that forms homotrimers on the surface of the virus and facilitates virulence through binding to angiotensin-converting enzyme 2 (ACE2) expressed on host cells. This protein is composed of two subunits S1 and S2. S1 subunit is responsible for binding to the host cell via ACE-2 receptor and S2 for fusion with the host cell. This antibody is specific for the S1 subunit of SARS-CoV-2 and was derived, sequenced and expressed from patients that had recovered from COVID-19 infection.
100μg
Verified Reactivity: SARS-CoV-2
Antibody Type: Recombinant
Host Species: Human
Immunogen: SARS-CoV-2
Formulation: 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Prior to use, quick spin the antibody vial to ensure recovery of maximum volume. Antibody can be stored in single use aliquots in -20°C for 2 years. Avoid repeated freeze and thaws.
Application: WB - Quality tested Direct ELISA - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. Recommended usage concentration for direct ELISA application is 93.6 ng/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone name AM004414 is also referred to as clone 414-4.
Application References(PubMed link indicates BioLegend citation): 1. Wan J. et al. 2020. Cell Rep. 32(3):107918
RRID: AB_2888747 (BioLegend Cat. No. 940401) AB_2888747 (BioLegend Cat. No. 940402)
Structure: Spike glycoprotein is a homotrimer. Each monomer consists of 1,273 a.a with a molecular weight of approximately 141 kD and consists of S1 and S2 subunits. S1 subunit is 673 a.a. long.
Distribution: Viral envelope protein, host cell membrane, host cell endoplasmic reticulum-Golgi intermediate compartment membrane
Function: Host cell surface receptor binding
Interaction: ACE2
Ligand/Receptor: ACE2
Biology Area: COVID-19
Antigen References: 1. Walls A.C. et al. 2020. Cell 181(2):281-292 2. Yan R. et al. 2020 Science 367 (6485):1444-1448 3. Wrapp D. et al. 2020 Science 367 (6483):1260-126 4. Shang J.et al. 2020 PNAS 117(21):11727-11734
Gene ID: 43740568
UniProt: View information about Viral Protein on UniProt.org
Clone: AM004414
Regulatory Status: RUO
Other Names: Spike glycoprotein, S glycoprotein (E2)
Isotype: Human IgG1, κ