Biolegend, 940502, Purified anti-SARS-CoV-2 S Protein S1 Recombinant Antibody, 100μg

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a single- stranded RNA virus that belongs in a family of viruses known as coronaviruses.   SARS-CoV-2 infection, known as COVID-19, was declared a pandemic by WHO on March 11, 2020 and among other symptoms leads to respiratory infection, pulmonary failure which can be fatal. SARS-CoV-2 is structurally composed of 4 main proteins (e.g. spike glycoprotein, envelope glycoprotein, membrane glycoprotein and nucleocapsid protein) and several accessory proteins. The spike glycoprotein (S) is a transmembrane molecule that forms homotrimers on the surface of the virus and facilitates virulence through binding to angiotensin-converting enzyme 2 (ACE2) expressed on host cells. This protein is composed of two subunits S1 and S2. S1 subunit is responsible for binding to the host cell via ACE-2 receptor and S2 for fusion with the host cell. This antibody is specific for the S1 subunit of SARS-CoV-2 and was derived, sequenced and expressed from patients that had recovered from COVID-19 infection.
100μg
Verified Reactivity: SARS-CoV-2
Antibody Type: Recombinant
Host Species: Human
Immunogen: SARS-CoV-2
Formulation: 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Prior to use, quick spin the antibody vial to ensure recovery of maximum volume. Antibody can be stored in single use aliquots in -20°C for 2 years. Avoid repeated freeze and thaws.
Application: ELISA Detection – Quality tested Direct ELISA – Verified
Recommended Usage: Recommended usage concentration for direct ELISA application is 7.1 ng/mL.
Application Notes: Clone name AM002414 is also referred to as clone 414-2.
Application References(PubMed link indicates BioLegend citation): 1. Wan J. et al. 2020. Cell Rep. 32(3):107918
RRID: AB_2888746 (BioLegend Cat. No. 940501) AB_2888746 (BioLegend Cat. No. 940502)
Structure: Spike glycoprotein is a homotrimer. Each monomer consists of 1,273 a.a with a molecular weight of approximately 141 kD and consists of S1 and S2 subunits. S1 subunit is 673 a.a. long.
Distribution: Viral envelope protein, host cell membrane, host cell endoplasmic reticulum-Golgi intermediate compartment membrane
Function: Host cell surface receptor binding
Interaction: ACE2
Ligand/Receptor: ACE2
Biology Area: COVID-19
Antigen References: Walls AC, et al. 2020. Cell. 181(2):281-292. Yan R, et al. 2020. Science. 367 (6485):1444-1448. Wrapp D, et al. 2020. Science. 367 (6483):1260-126. Shang J, et al. 2020. PNAS. 117(21):11727-11734.
Gene ID: 43740568
UniProt: View information about Viral Protein on UniProt.org
Clone: AM002414
Regulatory Status: RUO
Other Names: Spike glycoprotein, S glycoprotein (E2)
Isotype: Human IgG1, κ