Biolegend, 940902, Purified anti-SARS-CoV-2 Nucleocapsid Antibody, 100μg

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is a single stranded RNA virus that belongs in a family of viruses known as coronaviruses. SARS-CoV-2 infection, known as COVID-19, was declared a pandemic in 2020 and among other symptoms leads to respiratory infection, pulmonary failure which can be fatal. SARS-CoV-2 is structurally composed of 4 main proteins (e.g. spike glycoprotein, envelope glycoprotein, membrane glycoprotein and nucleocapsid protein) and several accessory proteins. The SARS-CoV-2 Nucleocapsid (N) is an abundant protein, that binds to the viral RNA and helps stabilize it. It has been proposed that N protein forms bimolecular condensates that both regulate viral replication and modulate the host response to the virus following infection. N protein is a major antigen that the host immune response is directed to following SARS CoV-2 infection making it a promising candidate for vaccine development and drug therapy.
100μg
Verified Reactivity: SARS-CoV-2
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: SARS-CoV-2 nucleocapsid recombinant protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested Direct ELISA - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For the Direct ELISA application, a concentration of 2.5 ng/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: SARS-CoV-2 is 90% homologous to SARS-CoV. Cross reactivity of anti-SARS-CoV-2 nucleocapsid antibody (clone A20087F) with SARS-CoV has not been tested.
RRID: AB_2888743 (BioLegend Cat. No. 940901) AB_2888743 (BioLegend Cat. No. 940902)
Structure: SARS-CoV-2 is a 419 amino acid protein with an expected molecular weight of 46 kD.
Distribution: Viral nucleoprotein, host cell Golgi apparatus
Function: RNA binding, transcription regulation
Biology Area: COVID-19
Antigen References: Zeng W, et al. 2020. Biochem Biophys Res Commun. 527:618-623. Yoshimoto FK. 2020. Protein J. 39:198-216. Cascarina SM & Ross ED. 2020. FASEB J. fj.202001351.
Gene ID: 43740575
UniProt: View information about Viral Protein on UniProt.org
Clone: A20087F
Regulatory Status: RUO
Other Names: Nucleocapsid phosphoprotein, N, Nucleoprotein
Isotype: Mouse IgG2b, κ