Biolegend, 941101, Purified anti-E2F1 Antibody, 25μg

E2F1 is a member of the E2F family of transcription factors, which is comprised of 6 members that display both activator and repressor functions. The primary functions of E2F1 are transcription activation and cell cycle regulation, namely in replication, DNA-damage response and repair, and apoptosis. E2F1 activation during G1 phase of cell cycle is vital for the transition into S phase and the subsequent replication and synthesis of DNA. Beyond its role in cell cycle control, E2F1 is a regulator of metabolism, primarily through β cell proliferation, insulin secretion, endocrine function, and adipogenesis. E2F1 activity is regulated through its binding to phosphorylated retinoblastoma proteins (pRB) and through acetylation when in its pRB-free form. E2F1 represents a critical target in cancer therapy. Through its stimulation of mTORC1 activity and tumor malignancy and growth, as well as its contribution to the Warburg effect by inducing glycolytic genes, E2F1 often behaves as an oncogene. Additionally, E2F1 can function as a tumor suppressor. Tumor-suppressive properties stem from its role in apoptosis through association with p53 and p73, as well as through inhibition of the NF-κB signaling pathway.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full-length recombinant human E2F1 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone was tested for ICC using HeLa cells fixed with 4% paraformaldehyde and permeabilized with either methanol or Triton X-100. Neither method resulted in E2F1 staining. This clone does not recognize mouse E2F1. However, when tested by western blot on mouse lysates, a ~35 kD protein of unknown origin was observed.
RRID: AB_2888772 (BioLegend Cat. No. 941101) AB_2888772 (BioLegend Cat. No. 941102)
Structure: E2F1 is a 437 amino acid protein with a predicted molecular weight of 47 kD.
Distribution: Ubiquitously expressed/Nucleus
Function: Transcription factor/Cell cycle
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Cell Death, DNA Repair/Replication, Transcription Factors
Molecular Family: Tumor Suppressors
Antigen References: Denechaud P, et al. 2017. Front. Endocrinol. 8:311. Liang Y, et al. 2015. Int. J. Oncol. 48:1650. Martinez-Balbas M, et al. 2000. Embo J. 19:662. Wu Z, et al. 2009. Int. J. Biochem. Cell Biol. 41:2389.
Gene ID: 1869
UniProt: View information about E2F1 on UniProt.org
Clone: E2F1/KH95
Regulatory Status: RUO
Other Names: PBR3, E2F Transcription Factor 1, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, RBAP-1, RBBP-3, PRB-Binding Protein E2F-1, Transcription Factor E2F1
Isotype: Mouse IgG2a, κ