Biolegend, 941203, Alexa Fluor® 647 anti-STAT4 Phospho (Tyr693) Antibody, 25tests

The signal transducer and activator of transcription (STAT) family of transcription factors are DNA-binding proteins required for transcriptional regulation of several cellular and physiological processes in response to cytokine and growth factor stimulation. Inactive STAT proteins are present as cytosolic monomers. C-terminal tyrosine phosphorylation by upstream Janus kinases results in STAT dimerization, translocation to the nucleus, and increased affinity for response elements found in the regulatory promoter of STAT target genes. In contrast to most other members of the STAT family, STAT4 expression is restricted to hematopoietic cells and responds to fewer cytokines, such as IL-12 and IL-2. STAT4, which requires phosphorylation at tyrosine 693 for activation, plays a pivotal role in the differentiation and proliferation of Th1 and Th17 cells, both of which are crucial effectors of chronic autoimmune disorders. In natural killer cells, STAT4 is required for normal development, maturation, and activation. Single nucleotide polymorphisms of STAT4 are implicated in the pathogenesis of systemic lupus erythematosis and rheumatoid arthritis, and are linked with increased risk of hepatocellular carcinoma.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide corresponding to human STAT4 phosphorylated at tyrosine 693
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: This antibody is predicted to recognize mouse STAT4 phosphorylated at tyrosine 694 and rat STAT4 phosphorylated at tyrosine 693 due to complete sequence homology between the immunizing peptide and the respective murine STAT4 orthologs.
RRID: AB_2936723 (BioLegend Cat. No. 941203) AB_2936723 (BioLegend Cat. No. 941204)
Structure: STAT4 is a 748 amino acid protein with predicted molecular weight of 86 kD.
Distribution: Hematopoietic cells/Nucleocytoplasmic localization
Function: Transcription factor, cell signaling
Biology Area: Cell Biology, Signal Transduction, Transcription Factors
Antigen References: Trinchieri G, et al. 2003. Nat. Rev. Immunol. 3:133. Remmers EF, et al. 2007. N. Engl J. Med. 357:977. Mukasa R, et al. 2010. Immunity. 32:616 Rapp M, et al. 2017. Sci. Immunol. 2:3296.
Gene ID: 6775
UniProt: View information about STAT4 Phospho Tyr693 on UniProt.org
Clone: A19016A
Regulatory Status: RUO
Other Names: Signal Transducer and Activator Of Transcription 4, SLEB11, Signal Transducer and Activator of Transcription 4 Variant 3
Isotype: Mouse IgG1, κ