Biolegend, 941501, Purified anti-SMARCA2 (BRM) Antibody, 25μg

SMARCA2 (also known as BRM) is a subunit of the SWI/SNF complex that facilitates chromatin remodeling. As a catalytic subunit, SMARCA2 contains an ATPase domain which uses ATP to alter DNA-histone interactions within a nucleosome. SMARCA2 as part of the chromatin remodeling complex regulates the expression of genes involved in cell cycle, metabolism, development and cancer. In most cases of cancer there is either downregulation or deficiency of the BRM gene. SMARCA2 serves as a target for anticancer therapy. Mutations in SMARCA2 can result in Nicolaides-Baraitser syndrome.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human SMARCA2 peptide
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone was tested for ICC using 4% PFA-fixed HeLa cells permeabilized with either methanol or Triton X-100. Neither method was compatible with SMARCA2 staining. This clone failed to enrich SMARCA2 from HeLa extracts when tested for IP.
RRID: AB_2890859 (BioLegend Cat. No. 941501) AB_2890859 (BioLegend Cat. No. 941502)
Structure: SMARCA2 is a 1,590 amino acid protein with a predicted molecular weight of 181 kD
Distribution: Ubiquitously expressed/Nucleus
Function: Chromatin Remodeling
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics
Antigen References: 1. Boris G. et al., 2014. Molecular and Cellular Biology. 34(6) 1136-1144 2. Euskirchen G. et al., 2012. J Biol Chem. 287(27) 30897-905 3. Van Houdt, J. et al., 2012. Nat Genet. 44 445-449 4. Vangamudi B. et al., 2015. Cancer Res. 75(18) 3865-3878
Gene ID: 6595
UniProt: View information about SMARCA2 on UniProt.org
Clone: W19134A
Regulatory Status: RUO
Other Names: HBRM, BRM, Probable Global Transcription Activator SNF2L2, ATP-Dependent Helicase SMARCA2, BRG1-Associated Factor 190B, Protein Brahma Homolog, Brahma Homolog, SNF2-Alpha, BAF190B, BAF190, SNF2L2, SNF2LA, HSNF2a, Sth1p, SNF2, SW12
Isotype: Rat IgG2b, κ