Biolegend, 942501, Purified anti-CTCF Antibody, 25μg

CTCF is an 83 kD transcription factor found in the nucleoplasm, which translocates to the nucleolus when the cell is undergoing cell differentiation. This protein can act as an insulator protein by binding to an unmethylated imprinting control region (ICR) structure and prevent the activation of cis-acting elements which in turn prevents gene activation. CTCF becomes further involved in gene regulation when it forms a complex with cohesin. This complex can stabilize the formation of a chromatin loop, which can promote or repress the binding of other transcription factors to the promoter and enhancer, leading to gene activation or gene repression respectively. CTCF has a role as a tumor suppressor, when the gene goes through a mutation or deletion there is a rise in variance in CpG methylation throughout the genome. This methylation contributes to the development of aggressive and invasive tumors which are seen in breast, endometrial and bladder cancers.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Synthetic human CTCF peptide
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 - 2.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone was tested for ICC on HeLa cells fixed with 4% PFA and permeabilized with either methanol or Triton X-100. Both methods resulted in high cytosolic background staining.
RRID: AB_2888905 (BioLegend Cat. No. 942501) AB_2888905 (BioLegend Cat. No. 942502)
Structure: CTCF is a 727 amino acid protein with a predicted molecular weight of 83 kD.
Distribution: Ubiquitously expressed/nucleus
Function: Transcription factor
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics, Transcription Factors
Antigen References: 1. Kim S, et al. 2015. Exp Mol Med. 47:e166. 2. Del Rosario BC, et al. 2019. Elife. 8:e42341. 3. Bell AC, et al. 2000. Nature. 405:482-5.
Gene ID: 10664
UniProt: View information about CTCF on UniProt.org
Clone: W19193A
Regulatory Status: RUO
Other Names: CCCTC-Binding Factor, Transcriptional Repressor CTCF, CFAP108
Isotype: Rat IgG2a, κ