Biolegend, 943704, Ultra-LEAF™ Purified anti-mouse CD191 (CCR1) Antibody, 1mg

CD191, also known as CCR1, is a 41 kD, G-protein coupled receptor expressed predominantly by monocytes. CCR1 is also expressed by a subset of T cells and eosinophils. CCR1 positive cells can migrate in response to a CCL3 and CCL5 gradient. CCR1 knock-out studies suggest that this molecule plays an important role in inflammation and susceptibility to viruses and parasites.
1mg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse CCR1 transfectants
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Neut - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by neutralization of the chemotaxis induced by recombinant mouse CCL5 (RANTES) (Cat. No. 594206) on mouse Baf3-mCCR1 transfectant chemoattractant cells. ND50 range: 0.4 - 2 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2910504 (BioLegend Cat. No. 943703) AB_2910504 (BioLegend Cat. No. 943704)
Structure: CD molecules, cytokine/chemokine receptors, GPCR
Distribution: Macrophages, monocytes, neutrophils, eosinophils, subset of T cells
Function: Chemotaxis, adhesion, regulating migration of cells in response to CCL3, CCL5, CCL7 and CCL23
Interaction: CCL15, Ga 14/16
Ligand/Receptor: CCL3, CCL5, CCL7 and CCL23
Cell Type: Eosinophils, Macrophages, Monocytes, Neutrophils
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Lionakis MS, et al. 2012. PLoS Pathog. 8:e1002865. 2. Gilliland CT, et al. 2013. J. Biol. Chem. 288:32194. 3. Gong X, et al. 1997. J. Biol. Chem. 272:11682. 4. Daugherty BL and Springer MS. 1997. Genomics. 41:294. 5. Pakianathan DR, et al. 1997. Biochemistry 36:9642.
Gene ID: 12768
UniProt: View information about CD191 on UniProt.org
Clone: S15040A
Regulatory Status: RUO
Other Names: CKR1, CKR-1, HM145, CMKBR1, MIP1aR, SCYAR1
Isotype: Rat IgG2b, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.