Biolegend, 943904, Alexa Fluor® 647 anti-EGR1 Antibody, 100tests

EGR1 (early growth response protein 1) is a zinc transcription factor vital to numerous physiological processes including cell cycle, differentiation, proliferation, metabolism, DNA damage response, and apoptosis. It also functions in mitogenesis, tissue repair, fibrosis, inflammatory response and immune response through its regulation of neutrophil gene expression and association with immune response genes such as TNFα, VEGF, and matrix metalloproteinases. In the central nervous system, EGR1 functions as an immediate early gene (IEG) and is vital to neuronal function and signaling, synaptic plasticity, cognition, and memory formation. Activated by acute mechanical injury and vascular stress, EGR1 regulates multiple cardiovascular pathological processes including atherosclerosis, intimal thickening and vascular repair in response to vascular injury, ischemia, angiogenesis, and cardiac hypertrophy. EGR1 is essential to the development, homeostasis, and repair of connective tissues through its regulation of genes of the extracellular matrix. EGR1 is a component of some oncogenic pathways and stimulates tumor growth and proliferation in prostate and gastric cancers. It also regulates a variety of tumor suppressors such as p53, PTEN, NAG-1, and TGFβ1, contributes to DNA repair, and induces apoptosis of tumor cells, thus functioning in dual roles as both an oncogene and as a tumor suppressor.
100tests
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human EGR1 protein
Formulation: µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: µg sizes: 0.5 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC (test sizes) - Quality tested ICC (µg size) - Quality tested
Recommended Usage: Each lot of the test-sized antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Each lot of the µg-sized antibody is quality control tested by immunocytochemistry. For immunocytochemistry using the ug size, a concentration range of 2.5 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: When using this clone for ICFC, we recommend using the True Phos perm buffer. We do not recommend using the True-Nuclear™ Transcription Factor Buffer Set or the Intracellular Staining Permeabilization Wash Buffer for ICFC testing due to poor EGR1 staining.
RRID: AB_2941638 (BioLegend Cat. No. 943903) AB_2941638 (BioLegend Cat. No. 943906) AB_2941638 (BioLegend Cat. No. 943904)
Structure: EGR1 is a 533 amino acid protein with a predicted molecular weight of 57 kD. The observed molecular weight ranges from 75 – 100 kD due to post-translational modifications.
Distribution: Ubiquitously expressed/ Nucleus and cytoplasm
Function: Transcription factor
Cell Type: Neurons
Biology Area: Cell Biology, Transcription Factors
Antigen References: Cullen EM, et al. 2010. Mol Immunol. 47:1701-9. Duclot F & Kabbaj M. 2017. Front Behav Neurosci. 11:35. Havis E & Duprez D. 2020. Int J Mol Sci. 21:1664. Khachigian LM. 2006. Circ Res. 98:186-91. Li T, et al. 2019. Med Oncol. 37:7.
Gene ID: 1958
UniProt: View information about EGR1 on UniProt.org
Clone: W16161B
Regulatory Status: RUO
Other Names: Early Growth Response 1, ZNF225, NGFI-A, TIS8, AT225, G0S30, KROX-24, ZIF-268, Transcription Factor ETR103
Isotype: Rat IgG2a, κ