Biolegend, 944501, Purified anti-Htra2/Omi Antibody, 25μg

Htra2/Omi (high temperature requirement 2) is a mitochondrial serine protease and member of the high-temperature requirement A family of oligomeric serine proteases that functions in the regulation of apoptosis, mitochondrial homeostasis, and protein quality control. Under normal conditions Htra2/Omi is localized to the mitochondria where it serves as a chaperone protein. Under stress conditions Htra2/Omi is released to the cytosol where it mediates apoptosis via proteolytic activity and disruption of caspase-IAP (inhibitors of apoptosis proteins) activity as well as through caspase-independent pathways. Loss or disruption of Htra2/Omi function results in mitochondrial defects and the aggregation of unfolded or misfolded proteins and has been implicated in various neurodegenerative disorders such as Parkinson’s disease, thus making Htra2/Omi an important potential therapeutic target in the treatment of such disorders. Likewise, due to its role as a regulator of cell death, Htra2/Omi may also be an important therapeutic target in the treatment of systemic sepsis and the prevention of Sepsis-Associated Encephalopathy (SAE) as well as a potential oncogenic target and modulator of chemotherapy-induced cytotoxicity.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: recombinant human Htra2 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone was tested for ICC on HeLa cells using three different fixation/permeabilization methods: methanol-only, PFA+ methanol, and PFA+ Triton X-100. Staining was only observed with PFA+ Triton X-100 permeabilization and even this was quite faint and only detected at high exposures. This clone is weakly reactive with mouse Htra2 by western blot.
RRID: AB_2890873 (BioLegend Cat. No. 944501) AB_2890873 (BioLegend Cat. No. 944502)
Structure: HTRA2 is a 458 amino acid protein with an expected molecular weight of 38 to 49 kD.
Distribution: Ubiquitous/ Mitochondria
Function: Protease, Hydrolase, Apoptosis
Antigen References: 1. Chen S, et al. 2018. Genes. 9:355. 2. Chien J, et al. 2009. Curr. Cancer Drug Targets. 9:451. 3. Hu Y, et al. 2019. Front. Mol. Neurosci. 12:48. 4. Zurawa-Janicka D, et al. 2013. Cell Stress Chaperones. 18:35.
Gene ID: 27429
UniProt: View information about Htra2/Omi on UniProt.org
Clone: 8G11-4B8-4F10-G5
Regulatory Status: RUO
Other Names: HTRA Serine Petidase 2, OMI, PRSS25, PARK13, High Temperature Requirement Protein A2, Serine Protease HTRA2 Mitochondrial, OMI Stress-Regulated Endoprotease, Serine Proteinase OMI, MGCA8
Isotype: Mouse IgG1, κ