Biolegend, 945501, Purified anti-ENO1 Antibody, 25μg

Enolase is highly conserved, multifunctional protein. In the cytoplasm, ENO1 acts as a metabolic enzyme and catalyzes 2-phosphoglycerate to phosphoenolpyruvate in the last step of the glycolytic cycle. Despite the lack of signal sequence in the ENO1 gene, ENO1 is found to be expressed on the surface of several hematopoietic cells though the transport mechanism is still unknown. Several of these cells include monocytes, T cells and B cells, and endothelial cells. On the cell surface, ENO1 acts as a plasminogen receptor and concentrates plasmin proteolytic activity at the pericellular area and protects plasmin from α2-antiplasmin inhibitors. In cancer cells, ENO1 promotes what is known as the Warburg effect, a modified cellular metabolism in which the tumor cells uptake excessive glucose and undergo glycolysis to produce lactate in both hypoxic conditions and normal oxygen levels. ENO1 also promotes tumor invasion through plasminogen activation and extracellular matrix degradation. Enolase can also be found in neurons and peripheral neuroendocrine cells. This neuron-specific enolase exists as ENO2 homodimer or an ENO1 and ENO2 heterodimer. Raised serum levels of these neuron-specific enolase have been found in all stages of neuroblastoma and it has been proposed that the plasmin formation caused by ENO1 enhances neuritogenesis. The ENO1 gene can also be alternatively spliced and produce a 37 kD protein named c-myc promoter-binding protein, or MBP-1. MBP-1 is localized to the nucleus and binds to the c-myc P2 promoter, preventing the transcription of proto-oncogenes.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human ENO1 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested KO/KD-WB, ICC, IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.05 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: When tested for ICC using 4%-PFA fixed HeLa cells permeabilized with methanol or Triton X-100, high non-specific staining in the nucleus was observed. We therefore do not recommend these fix-perm methods for this clone.
RRID: AB_2892513 (BioLegend Cat. No. 945501) AB_2892513 (BioLegend Cat. No. 945502)
Structure: ENO1 is a 434 amino acid protein with a predicted molecular weight of 47 kD.
Distribution: Cytosolic/Ubiquitously expressed
Function: Glycolytic enzyme
Biology Area: Cell Biology
Antigen References: Capello M, et al. 2011. FEBS J. 278:1064-74 Capello M, et al. 2016. Oncotarget. 7:5598-5612 Cappello P, et al. 2017. Front Biosci (Landmark Ed). 22:944-959 Díaz-Ramos A, et al. 2012. J Biomed Biotechnol. 2012:156795 Didiasova M, et al. 2019. Front Cell Dev Biol. 7:61 Isgrò MA, et al. 2015. Adv Exp Med Biol. 867:125-43. Liberti MV and Locasale JW. 2016. Trends Biochem Sci. 41:211-218.
Gene ID: 2023
UniProt: View information about ENO1 on UniProt.org
Clone: A18166G
Regulatory Status: RUO
Other Names: Enolase 1; Alpha-Enolase; 2-Phospho-D-Glycerate Hydro-Lyase; Plasminogen-Binding Protein; Phosphopyruvate Hydratase
Isotype: Mouse IgG2b, κ