Biolegend, 946101, Purified anti-SARS-CoV-2 Nucleocapsid Antibody, 25μg

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is a single stranded RNA virus that belongs in a family of viruses known as coronaviruses.   SARS-CoV-2 infection, known as COVID-19, was declared a pandemic in 2020 and among other symptoms leads to respiratory infection, pulmonary failure which can be fatal. SARS-CoV-2 is structurally composed of 4 main proteins (e.g. spike glycoprotein, envelope glycoprotein, membrane glycoprotein and nucleocapsid protein) and several accessory proteins. The SARS-CoV-2 Nucleocapsid (N) is an abundant protein, that binds to the viral RNA and helps stabilize it. It has been proposed that N protein forms bimolecular condensates that both regulate viral replication and modulate the host response to the virus following infection.  N protein is a major antigen that the host immune response is directed to following SARS CoV-2 infection making it a promising candidate for vaccine development and drug therapy.
25μg
Verified Reactivity: SARS-CoV-2
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: SARS-CoV-2 Nucleocapsid recombinant protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested Direct ELISA, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For Direct ELISA application, a concentration of 3.7 ng/mL is recommended. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: SARS-CoV-2 is 90% homologous to SARS-CoV. Cross reactivity of anti-SARS-CoV-2 Nucleocapsid antibody (clone A20087H) with SARS-CoV has not been tested. BioLegend offers two clones specific for SARS-CoV-2 nucleocapsid, clones A20087F and A20087H Both clones have been verified for western blot, with A20087F showing a higher affinity for SARS-CoV-2 nucleocapsid for this application Both clones have been verified for direct ELISA and displayed similar EC50 values for this application A20087H has been verified for ICFC, while A20087F is not recommended for this application
RRID: AB_2892515 (BioLegend Cat. No. 946101) AB_2892515 (BioLegend Cat. No. 946102)
Structure: SARS-CoV-2 is a 419 amino acid protein with an expected molecular weight of 46 kD
Distribution: Viral nucleoprotein, host cell Golgi apparatus
Function: RNA binding, transcription regulation
Antigen References: Zeng W. et al. 2020. Biochem Biophys Res Commun 527(3):618-623. Yoshimoto FK. 2020. Protein J. 39(3):198-216. Cascarina SM. & Ross ED. 2020. The FACEB Journal. 34:9832-9842.
Gene ID: 43740575
UniProt: View information about Viral Protein on UniProt.org
Clone: A20087H
Regulatory Status: RUO
Other Names: Nucleocapsid phosphoprotein, N, Nucleoprotein
Isotype: Mouse IgG2b, κ