Biolegend, 946602, Purified anti-LAT Phospho (Tyr171) Antibody, 100microl

LAT, or Linker for Activation of T cells, is a transmembrane protein expressed mainly in T cells and a limited number of other immune cells such as natural killer cells, mast cells, and immature B cells. As an adaptor protein, phosphorylated LAT facilitates the recruitment of other signaling proteins and functions as a nucleating site for multiprotein signaling complexes. These signaling complexes can propagate TCR, or T Cell antigen Receptors, and activate downstream effectors, which trigger T cell proliferation or cytokine expression. LAT (Tyr171) is one of the nine tyrosines in LAT conserved between human, mice, and rat. LAT (Tyr171) is necessary for the activation of PI 3-kinase, a protein that regulates the recruitment of other proteins that facilitate in T-cell receptor signal transduction such as PLC-gamma1 and Sos1. When LAT (Tyr171) and (Tyr191) is phosphorylated by ZAP-70, Gads can bind directly to LAT (Tyr171) and (Tyr191) and mediate the recruitment of SLP-76.
100microl
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide corresponding to human LAT phosphorylated at Tyr 171
Formulation: Phosphate Buffer, 0.5% BSA, 0.09% NaN3
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.05 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested dilution of this reagent is 1000 - 1:5000. For immunocytochemistry, a dilution of 1:10 is recommended. For intracellular flow cytometric staining, the suggested dilution of this reagent is 1:20 per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: When this clone is used in WB at a dilution lower than 1:1000 it may show non-specific bands at 50 kD and above. This clone has been tested by ICC on H2O2 treated and untreated Jurkat, with three fix and permeabilization methods (100% methanol, 4% PFA plus methanol and 4% PFA plus Triton X-100). All three methods can be used for ICC staining, but the PFA plus Triton X-100 fixation/permeabilization method produced the best staining. This clone has only been tested on True-PhosTM perm buffer (Cat. No. 425401) by ICFC
RRID: AB_2904456 (BioLegend Cat. No. 946601) AB_2904456 (BioLegend Cat. No. 946602)
Structure: LAT is a 262 amino acid protein with a predicted molecular weight of 36-38 kD.
Distribution: Plasma membrane/T Cells
Function: Plasma membrane/T Cells
Cell Type: Mast cells, NK cells, T cells
Biology Area: Cell Biology, Immunology, Signal Transduction
Molecular Family: Phospho-Proteins
Antigen References: Balagopalan L. et al., 2010, Cold Spring Harb Perspect Biol., 2(8): a005512 Balagopalan L. et al., 2020, PLoS One, 15(2): e0229036 Lin J. and Weiss A., 2001, J Biol Chem., 276(31): 29588-95 Paz et. al., 2001, Biochem J., 356, 461-71 Zhang W. et al., 2000, J Biol Chem., 275(30): 23355-61
Gene ID: 27040
UniProt: View information about LAT Phospho Tyr171 on UniProt.org
Clone: A20005D
Regulatory Status: RUO
Other Names: Linker For Activation Of T Cells; P36-38
Isotype: Mouse IgG2b, κ