Biolegend, 946606, PE anti-LAT Phospho (Tyr171) Antibody, 100tests

LAT, or Linker for Activation of T cells, is a transmembrane protein expressed mainly in T cells and a limited number of other immune cells such as natural killer cells, mast cells, and immature B cells. As an adaptor protein, phosphorylated LAT facilitates the recruitment of other signaling proteins and functions as a nucleating site for multiprotein signaling complexes. These signaling complexes can propagate TCR, or T Cell antigen Receptors, and activate downstream effectors, which trigger T cell proliferation or cytokine expression. LAT (Tyr171) is one of the nine tyrosines in LAT conserved between human, mice, and rat. LAT (Tyr171) is necessary for the activation of PI 3-kinase, a protein that regulates the recruitment of other proteins that facilitate in T-cell receptor signal transduction such as PLC-gamma1 and Sos1. When LAT (Tyr171) and (Tyr191) is phosphorylated by ZAP-70, Gads can bind directly to LAT (Tyr171) and (Tyr191) and mediate the recruitment of SLP-76.
100tests
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide corresponding to human LAT phosphorylated at Tyr 171
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: When this clone is used in WB at a dilution lower than 1:1000 it may show non-specific bands at 50 kD and above. This clone has been tested by ICC on H2O2 treated and untreated Jurkat, with three fix and permeabilization methods (100% methanol, 4% PFA plus methanol and 4% PFA plus Triton X-100). All three methods can be used for ICC staining, but the PFA plus Triton X-100 fixation/permeabilization method produced the best staining. This clone has only been tested on True-PhosTM perm buffer (Cat. No. 425401) by ICFC
RRID: AB_2936765 (BioLegend Cat. No. 946605) AB_2936765 (BioLegend Cat. No. 946606)
Structure: LAT is a 262 amino acid protein with a predicted molecular weight of 36-38 kD.
Distribution: Plasma membrane/T Cells
Function: Plasma membrane/T Cells
Cell Type: Mast cells, NK cells, T cells
Biology Area: Cell Biology, Immunology, Signal Transduction
Molecular Family: Phospho-Proteins
Antigen References: Balagopalan L. et al., 2010, Cold Spring Harb Perspect Biol., 2(8): a005512 Balagopalan L. et al., 2020, PLoS One, 15(2): e0229036 Lin J. and Weiss A., 2001, J Biol Chem., 276(31): 29588-95 Paz et. al., 2001, Biochem J., 356, 461-71 Zhang W. et al., 2000, J Biol Chem., 275(30): 23355-61
Gene ID: 27040
UniProt: View information about LAT Phospho Tyr171 on UniProt.org
Clone: A20005D
Regulatory Status: RUO
Other Names: Linker For Activation Of T Cells; P36-38
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.