Biolegend, 946702, Purified anti-MOB1A Antibody, 100μg

MOB1 was first identified in yeast. MOB1 belongs to the Mps One Binder (MOB) co-activator protein family and is highly conserved from yeast to mammals. In humans, there are two genes that are highly homologous to yeast's MOB1: MOB1A and MOB1B. At the protein level, MOB1A and MOB1B are 95% identical. MOB1 is mainly located in the cytoplasm and is part of the Hippo pathway. The Hippo pathway was first identified in Drosophilia and is highly conserved in mammals. The pathway regulates cell contact inhibition, organ size control, and cancer development. In mammals, MOB1 is phosphorylated by MST1/2 and the activated MOB1 promotes LATS1/2 activation which in turn phosphorylates YAP1 and TAZ, causing them to be retained in the cytoplasm for ubiquitous degradation.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human MOB1A protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone is not suitable for ICC. It failed to produce specific signal by ICC after fixing cells following three fix and perm methods (e.g. 4% PFA/methanol, methanol or 4% PFA/Triton X-100).
RRID: AB_2894533 (BioLegend Cat. No. 946701) AB_2894533 (BioLegend Cat. No. 946702)
Structure: MOB1A is a 216 amino acid protein with a predicted molecular weight of 25 kD.
Distribution: Nuclear and cytosolic
Function: Hippo pathway
Biology Area: Cell Biology
Antigen References: Florindo C. et al. 2012. J Cell Sci. 125:3085-90. Hergovich A. 2011. Cell Signal. 23:1433-40. Hong A. et al. 2016. Nat Rev Gastroenterol Hepatol. 13:324-37. Stavridi ES. et al. 2003. Structure. 11:1163-70. Wilmeth L. et al. 2010. Mol Biol Cell. 21:380-92. Zeng Q. and Hong W. 2008. Cancer Cell. 13:188-92.
Gene ID: 55233
UniProt: View information about MOB1A on UniProt.org
Clone: W20004B
Regulatory Status: RUO
Other Names: MOB Kinase Activator 1A
Isotype: Rat IgG2b, κ