Biolegend, 947206, Alexa Fluor® 647 anti-FOXO1 Antibody, 100tests

Forkhead box protein O1 (FOXO1) is a member of the Forkhead box class O family of transcription factors. As such, it is involved in the regulation of a wide range of vital cellular processes including cell cycle progression, differentiation, proliferation, DNA repair, stress response, and apoptosis. FOXO1 is activated via serine and threonine phosphorylation. Phosphorylation is triggered by external stimuli including growth factors, cytokines, and insulin and can be PKB/Akt-mediated as part of the P13K-Akt/SGK pathway on Thr24, Ser256, and Ser319, mediated by CDK2 or CK1 on Ser249 and Ser322/325, respectively, or induced by serum and glucocorticoid-inducible kinase or NFkB kinase. Upon phosphorylation, FOXO1, which is localized to the nucleus, translocates to the cytoplasm following binding to 14-3-3 proteins. In addition to phosphorylation, FOXO1 can be activated via acetylation of its lysine residues by coactivator p300. FOXO1 is vital to the regulation of metabolism through the promotion of glucose homeostasis and gluconeogenic enzyme expression, adipose tissue and skeletal muscle formation, and oxidative stress regulation. FOXO1 may inhibit cell cycle progression and induce apoptosis in cancer cells.
100tests
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human FOXO1 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: This clone was tested for ICC using serum-starved HeLa cells treated with insulin and untreated serum-starved HeLa cells. Staining was expected to localize to the nucleus prior to treatment and localize to the cytoplasm following treatment. Three fix/perm methods were used: methanol fixation or 4% PFA fixation followed by permeabilization with either methanol or Triton X-100. Only PFA fixation followed by methanol permeabilization produced a strong signal and the expected shift in localization. This clone was tested for IHC on human melanoma tissue using Biolegend’s DAB Chromagen. No staining was produced.
RRID: AB_2927910 (BioLegend Cat. No. 947205) AB_2927910 (BioLegend Cat. No. 947206)
Structure: FOXO1 is a 655 amino acid protein with a predicted molecular weight of 69 kD.
Distribution: Ubiquitously expressed /Nucleus and Cytoplasm
Function: Transcription Factor
Interaction: RET signaling: PDGF, EGFR, DAP12, MAPK, NGF Cytokine Receptor Gamma-Chain Family signaling: IL-21, IL-7, IL-9, IL-15, IL-2, IL-4, adipokines, insulin, thrombopoietin GAB1 signalosome: PI3K/AKT, GAB1 Immune response IL-23 signaling: Tie2
Cell Type: B cells, Tregs
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: Greer E and Brunet A. 2005. Oncogene. 24:7410. Gross D, et al. 2008. Oncogene. 27:2320. Perrot V and Matthew R. 2005. Mol. Endocrinol. 19:2283. Tzivion G, et al. 2011. BBA. 1813:1938. Yang Y, et al. 2005. EMBO. 24:1021.
Gene ID: 2308
UniProt: View information about FOXO1 on UniProt.org
Clone: W20064D
Regulatory Status: RUO
Other Names: Forkhead Box O1, FOXO1A, FKH1, FKHR
Isotype: Rat IgG2b, κ