Biolegend, 947406, Alexa Fluor® 647 anti-human cdc2 Phospho (Thr14) Antibody, 100tests

Cyclin-dependent kinase 1 (Cdk1), also known as cell division control protein 2 (cdc2), is a key regulatory kinase essential for cell growth, proliferation, cell cycle progression, and early embryonic development. It is responsible for driving cells through G2 phase into M phase. Prior to mitosis, cdc2, along with its binding partner cyclin B1, exists in an inactive state when phosphorylated on Thr14 and Tyr15 by PKMYT1 (MYT1) and WEE1. This B1-cdc2 complex is activated during G2 and early mitosis by cdc25-mediated dephosphorylation. During mitosis, cdc2 inhibits DNA re-replication and subsequent polyploidy and cell senescence through the sequestering of cyclin A2 and suppression of the formation of CDK2-cyclin A complexes. cdc2 can function as a substitute for other cdc family members and drive the progression of the cell cycle in the event of their loss. cdc2 functions as a positive regulator of global translation, protein synthesis, and genome stability and participates in DNA damage repair, checkpoint activation, and DNA replication fork progression. It is overexpressed in several cancers including liver, breast, and colorectal cancers and has been implicated in the promotion of tumorigenesis.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide corresponding to human cdc2 phosphorylated at threonine 14
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: This clone is predicted to react with mouse cdc2 when phosphorylated at threonine 14 due to complete sequence homology between immunizing peptide and the cdc2 mouse orthologue. This clone was tested for ICC using lambda protein phosphatase analysis and HeLa cells fixed with methanol or 4% PFA followed by permeabilization with either methanol or Triton X-100. Only PFA fixation followed by methanol permeabilization produced phospho-specific staining of cdc2.
RRID: AB_2936756 (BioLegend Cat. No. 947405) AB_2936756 (BioLegend Cat. No. 947406)
Structure: cdc2 is a 297 amino acid protein with a predicted molecular weight of 34 kD.
Distribution: Ubiquitously expressed/ Mitochondria, nucleus, cytoskeleton, cytoplasm
Function: Serine/threonine-protein kinase/ Cell Cycle
Interaction: Cyclin B1, Cyclin A, PKMYT1 (MYT1)
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Signal Transduction
Molecular Family: Protein Kinases/Phosphatase
Antigen References: Chow J, et al. 2011. Mol. Cell Biol. 31:1478-91. Diril M, et al. 2012. PNAS. 109:3826-31. Haneke K, et al. 2020. J. Cell. Biol. 219: e201906147. Liao H, et al. 2017. Oncotarget. 8:90662-73. Prevo R, et al. 2018. Cell Cycle. 17:1513-23.
Gene ID: 983
UniProt: View information about cdc2 on UniProt.org
Clone: A20004B
Regulatory Status: RUO
Other Names: Cyclin Dependent Kinase 1, Cdk1, CDC28A, P34CDC2, P34 Protein Kinase
Isotype: Mouse IgG2b, κ