Biolegend, 947808, PE anti-human CD273 (B7-DC, PD-L2) Antibody, 100tests

PD-L2 is a type I transmembrane protein of 273 amino acids, and it is a member of the B7 family. Human PD-L2 has 70% amino acid identity to its mouse orthologue. PD-L2 is the second ligand for PD-1, and its binding to PD-1 leads to the inhibition of T cell receptor–mediated lymphocyte proliferation and cytokine secretion. PD-L2/PD-1 interaction suppresses immune responses against autoantigens and tumors and plays an important role in the maintenance of peripheral immune tolerance. Antibody blocking of PD-L2 on dendritic cells results in enhanced T cell proliferation and cytokine production. Interaction of PD-1 by PD-L1-Ig or PD-L2-Ig fusion protein has an inhibitory effect on proliferation and cytokine production during anti-CD3-mediated stimulation. PD-L2 is expressed in biopsies from human asthmatics and lungs of aeroallergen-treated mice and its expression correlates with the asthma severity. In vivo blockade of PD-L2 reduces the severity of allergen-driven airway hyperresponsiveness (AHR).  Blockage of PD-L2 induces the IL-12 production by DC; IL-12 may limit the severity of allergen-induced AHR antagonizing IL-13. In addition, blockade of PD-1 does not have effect on AHR; therefore, PD-L2 controls asthma severity in a PD-1 independent manner. PD-L2 binds to a second receptor, RGMb (repulsive guidance molecule b) which is expressed in lung interstitial macrophages and alveolar epithelial cells and is a co-receptor for bone morphogenetic proteins BMP2/4. Blockade of the RGMb/PD-L2 interaction significantly impaired the development of respiratory tolerance since the initial T cell expansion is inhibited.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human PD-L2 (B7-DC, CD273)-Fc chimera
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2941640 (BioLegend Cat. No. 947807) AB_2941640 (BioLegend Cat. No. 947808)
Structure: Dimer
Distribution: Immature dendritic cells, mature dendritic cells, subset of macrophages, placental endothelium, and medullary thymic epithelial cells
Function: Inhibits T cell mediated proliferation and cytokine production by CD4+ T cells. Regulate T cell responses. PD-L2 expression is up-regulated on antigen-presenting cells by IFN-γ.
Interaction: Antigen-stimulated T and B cells, regulatory T cells, follicular T and B cells, dendritic cells, monocytes
Ligand/Receptor: PD-1 (CD279), RGMb
Cell Type: Antigen-presenting cells, Dendritic cells, Epithelial cells, Macrophages, Plasma cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Proliferation and Viability, Immunology
Molecular Family: CD Molecules, Immune Checkpoint Receptors, Soluble Receptors
Antigen References: Latchman Y, et al. 2001. Nat. Immunol. 2:261. Brown JA, et al. 2003. J. Immunol. 170:1257. Youngnak P, et al. 2003. Biochem. Biophys. Res. Commun. 307:672. Singh AK, et al. 2011. Allergy. 66:155. Lewkowich IP, et al. 2013. Mucosal. Immunol. 6:728. Xiao Y, et al. 2014. J. Exp. Med. 211:943. Bardhan K, et al. 2016. Front Immunol. 7:550.
Gene ID: 80380
UniProt: View information about CD273 on UniProt.org
Clone: A20050B
Regulatory Status: RUO
Other Names: CD273, Programmed cell death 1 ligand 2 (PD-L2), B7 dendritic cell molecule (B7-DC), Butyrophilin B7-DC, PDCD1 Ligand 2
Isotype: Rat IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.