Biolegend, 948606, Alexa Fluor® 647 anti-GATA4 Antibody, 100tests

GATA binding protein 4 (GATA4) is a zinc-finger transcription factor that plays a crucial role in transcriptional activation and cell growth, differentiation, survival, and senescence. It is essential to cardiac development and function. Along with GATA6, it modulates cardiac stress response and regulates cardiomyocyte formation, replication, and function. GATA4 participates in second heart field transcription and differentiation and ventricular morphogenesis through downstream regulation of Mef2c and Hand2. Mutations in GATA4 can result in myocardial abnormalities, myocardial hypoplasia, absence of a proepicardium, abnormal valve formation, and endocardial cushion defects. In the intestine, GATA4, along with GATA6, is also responsible for regulating epithelial cytodifferentiation and structure, and for maintaining balance between enterocytes and secretory cells through the coordination of Notch signaling. GATA4 plays a vital role in human testicular development through its regulation of the expression of steroidogenic factors StAR, INHA, HSD3B2, and CYP19A1 and sex-determining genes SRY, AMH, and SOX9.
100tests
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human GATA4 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: This clone was tested for ICC using HeLa cells and SH-SY5Y cells fixed with 4% paraformaldehyde and permeabilized with either methanol or Triton X-100. Both methods resulted in specific, but weak GATA4 staining. We therefore do not recommend this clone for the application. While this clone detected GATA4 by WB, it also showed a very weak affinity for the protein. Because of this, we do not recommend this clone for the application. This clone failed IHC testing due to lack of specificity for GATA4. We therefore do not recommend this clone for the application. This clone is not compatible with our True Nuclear™ Transcription Factor and Cyto-Fast™ buffer sets. We recommend using using True-Phos™ Perm Buffer (Cat. No. 425401) for ICFC.
RRID: AB_2924630 (BioLegend Cat. No. 948605) AB_2924630 (BioLegend Cat. No. 948606)
Structure: GATA4 is a 442 amino acid protein with a predicted molecular weight of 44 kD
Distribution: Expressed in the heart, intestine, liver, endoderm, gonads/ Nucleus
Function: Transcription factor
Biology Area: Cell Biology, Transcription Factors
Antigen References: Lourenco D, et al. 2011. Proc Natl Acad Sci. USA. 4:1597-602. Walker E, et al. 2014. Dev Biol. 392:283-94. Zhou P, et al. 2012. Curr Top Dev Biol. 100:143-69.
Gene ID: 2626
UniProt: View information about GATA4 on UniProt.org
Clone: W19078B
Regulatory Status: RUO
Other Names: GATA-binding factor 4, GATA-4, TACHD, ASD2, VSD1, TOF
Isotype: Rat IgG2a, κ