Biolegend, 949203, Ultra-LEAF™ Purified anti-human BMP-14 (GDF-5) Antibody, 100μg

Growth and differentiation factor-5 (GDF-5) was initially identified in partially purified extracts from newborn calf articular cartilage. It is part of the bone morphogenetic protein (BMP) family of proteins, which are members of the TGF-β superfamily and plays a key role in skeletal development, bone formation, and stem cell differentiation. Human GDF-5 is highly homologous to its murine equivalent and the mature proteins differ only by one amino acid. GDF-5 belongs together with GDF-6 and GDF-7 to a subfamily of the BMPs. BMPs signal through heterotetrameric complex receptors, which include four type I receptors (ALK1, ALK2, ALK3, and ALK6) and three type II receptors (BMPRII, ActRIIA, and ActRIIB). GDF-5 binds specifically to BMPR1b, BMPRII, and ACTRIIA receptors forming a heterodimeric complex thus inducing the phosphorylation of smad1, 5, 8 and the translocation to the nucleus. GDF-5 enhances endochondral bone growth, development of limb skeletons and joints, and odontogenesis. GDF-5 stimulates osteogenic differentiation and increases VEGF levels in fat-derived stromal cells in vitro. In addition, GDF-5 stimulates the proteoglycans and type II collagen production in intervertebral disc cells, and enhances cell proliferation and matrix synthesis in annulus fibrosus and nucleus pulposus cells, which inhibits degenerative disc disease. GDF-5 could be suitable for gene therapy for lumbar intervertebral disc degeneration. Mutations in the human GDF-5 gene result in a broad spectrum skeletal disorders. GDF-5 is a potent neurotrophic factor in vitro and in vivo for rat dopaminergic midbrain neurons, specifically the ones that degenerate in Parkinson's disease. Exogenous GDF-5 is neuroprotective in animal models of Parkinson’s disease. Moreover, it is a key regulator of the growth and elaboration of pyramidal cell dendrites in the developing hippocampus.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human BMP-14 (GDF-5)
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Neut - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by neutralizing the differentiation induced by recombinant human BMP-14 (GDF-5) (Cat. No. 779506) at 1.5 µg/mL on ATDC5 mouse chondrogenic cells. ND50 range: 3 – 15 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2910514 (BioLegend Cat. No. 949203) AB_2910514 (BioLegend Cat. No. 949204)
Structure: Homodimer
Distribution: Predominantly expressed in cartilaginous tissues, precartilaginous mesenchymal condensation, and throughout the cartilaginous cores of the developing long bones
Function: Skeletal development during embryogenesis, joint formation, tendon repair, and odontogenesis. MicroRNA-21 suppresses the expression of GDF-5 by inducing GDF-5 mRNA decay in chondrocytes. Noggin inhibits GDF-5.
Interaction: Chondrocytes, osteoblast, periosteum cells, fibroblasts, hippocampal pyramidal cells, and dopaminergic midbrain neurons
Ligand/Receptor: BMPR1b, BMPRII, and ACTRIIA
Cell Type: Embryonic Stem Cells, Fibroblasts, Hematopoietic stem and progenitors, Mesenchymal cells, Mesenchymal Stem Cells, Neural Stem Cells, Neurons
Biology Area: Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: Chang SC, et al. 1994. J Biol Chem. 269:28227-34. Hotten G, et al. 1994. Biochem Biophys Res Commun. 204:646-52. Nishitoh H, et al. 1996. J Biol Chem. 271:21345-52. Groppe J, et al. 2002. Nature. 420:636-42. Chen X, et al. 2006. J Mol Cell Cardiol. 41:26-33. Kleinschmidt K, et al. 2013. Biomaterials. 34:5926-36. Osorio C, et al. 2013. Development. 140:4751-62. Zhang Y, et al. 2014. Exp Mol Med. 46:e79. Li YF, et al. 2015. Int J Clin. Exp Pathol. 8:719-26.
Gene ID: 8200
UniProt: View information about BMP-14 on UniProt.org
Clone: A16098C
Regulatory Status: RUO
Other Names: Bone morphogenetic protein 14 (BMP14), cartilage-derived morphogenetic protein 1 (CDMP1), BDA1C, Lipopolysaccharide associated protein 4 (LAP4), LPS associated protein 4, OS5, SYM1B, SYNS2, GDF5
Isotype: Mouse IgG2b, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.