Biolegend, 949303, PE anti-Syk Phospho (Tyr296) Recombinant Antibody, 25tests

Syk is a non-receptor tyrosine kinase that is a member of the Syk family of tyrosine kinases, with broad expression in hematopoietic cells. Upon immune receptor engagement, phosphorylated ITAM motifs within the cytosolic region of the activated receptor become phosphorylated and recruit Syk to the activated receptor via SH2 domains. ITAM-bound Syk is catalytically active, transmitting signals to multiple downstream signaling partners that drives processes such as phagocytosis, proliferation, and differentiation. Recent evidence also suggests a role for Syk in non-immune cells. Elevated Syk activation is associated with B-cell malignancies, rheumatoid arthritis, and Alzheimer’s disease, and multiple Syk inhibitors are being developed in clinical trials. Syk activity and function are both regulated by tyrosine phosphorylation at multiple sites. Tyr323 and Tyr352 phosphorylation facilitate binding to downstream effectors, and phosphorylation of Tyr525/Tyr526 in the activation loop is required for kinase activity. Tyr296 phosphorylation has been reported in lung epithelial cancer cells, and has been shown to occur as part of an autophosphorylation mechanism in vitro . This site is embedded within a larger N-terminal linker region that is required for nuclear localization in breast cancer cells. Despite rapid autophosphorylation of this site upon Syk activation, it is not required for Syk activity.
25tests
Verified Reactivity: Human, Mouse
Antibody Type: Recombinant
Host Species: Mouse
Immunogen: Synthetic peptide corresponding to Syk phosphorylated at tyrosine 296
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: When Syk is phosphorylated at tyrosine 296, the protein may display a slightly higher observed molecular weight by western blot.
RRID: AB_3716900 (BioLegend Cat. No. 949303) AB_3716900 (BioLegend Cat. No. 949304)
Structure: Syk isoform A is a 635 amino acid protein with a predicted molecular weight of 72 kD. Syk isoform B is a 612 amino acid protein with a predicted molecular weight of 69.5 kD. Syk isoform B lacks the Tyr296 site.
Distribution: Plasma membrane
Function: B-cell receptor, integrin signaling
Biology Area: Cell Biology, Immunology, Signal Transduction
Molecular Family: Protein Kinases/Phosphatase
Antigen References: Mansueto MS, et al. 2019. J Biol Chem. 294:7658-7668. Geahlen RL. 2009. Biochim Biophys Acta. 1793:1115-27. Taniguchi T, et al. 1991. J Biol Chem. 266:15790-6. Toyabe S, et al. 2001. Immunology. 103:164-71.
Gene ID: 6850
UniProt: View information about Syk Phospho on UniProt.org
Clone: QA20B02
Regulatory Status: RUO
Other Names: Spleen Associated Tyrosine Kinase, Spleen Tyrosine Kinase, Tyrosine-Protein Kinase Syk, IMD82
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.