Biolegend, 949601, Purified anti-mouse CD44 Antibody, 25μg

CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant mouse CD44 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested ICC, IHC-F - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. For immunohistochemical staining on frozen tissue sections, a concentration range of 1.125 - 5.0 µg/mL is suggested It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Not recommended for western blotting. Not reactive for human CD44 (tested in WB and IHC-P).
RRID: AB_2922650 (BioLegend Cat. No. 949601) AB_2922650 (BioLegend Cat. No. 949602)
Structure: Variable splicing of CD44 gene generates many CD44 isoforms, 80-95 kD
Distribution: All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells
Function: Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
Ligand/Receptor: Hyaluronan, MIP-1β, fibronectin, collagen
Cell Type: B cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal cells
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. Haynes BF, et al. 1991. Cancer Cells. 3:347-50. Goldstein LA, et al. 1989. Cell. 56:1063-72. Mikecz K, et al. 1995. Nat Med. 1:558-63. Hegde V, et al. 2008. J Leukoc Biol. 84:134-42. Liu T, et al. 2009. Biol Direct. 4:40.
Gene ID: 960
UniProt: View information about CD44 on UniProt.org
Clone: W19210B
Regulatory Status: RUO
Other Names: Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
Isotype: Rat IgG2a, κ