Biolegend, 949902, Purified anti-CD248 Antibody, 100μg

CD248, or TEM1, is classified as a C-type lectin-like transmembrane glycoprotein localized in the plasma membrane. CD248 is expressed mainly during embryonic development. In normal human adults, its expression is retained in very low levels in endometrial stroma, bone marrow fibroblasts, and corpus luteum. In adults with cancer, CD248 can be found to be expressed in tumor stroma and endothelium. Fibronectin and collagen I/IV have been reported to be ligands of CD248. Through this interaction, tumor cells overexpressing CD248 have increased cellular migration and adhesive properties, which in turn may support tumor progression and invasion. Mac-2 BP/90K has also been identified as a CD248 ligand and data suggests that CD248-Mac2 BP/90K interactions may also play an important role during tumor progression and metastasis.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide for human CD248
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ICC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1 - 5 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone is not suitable for WB. This clone does not stain formalin-fixed paraffin- embedded tissue by IHC-IF.
Product Citations: Lu T, et al. 2023. Cancer Immunol Immunother. :. PubMed
RRID: AB_2910519 (BioLegend Cat. No. 949901) AB_2910519 (BioLegend Cat. No. 949902)
Structure: CD248 is a 757 amino acid protein with a predicted molecular weight of 81 kD.
Distribution: Plasma membrane
Function: Embryonic development
Ligand/Receptor: Fibronectin; collagen I/IV; Mac-2 BP/90K
Biology Area: Cell Adhesion, Cell Death, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability
Antigen References: Becker R, et al. 2008. FASEB J. 22:3059-67. MacFadyen JR, et al. 2005. FEBS Lett. 579:2569-75. Teicher B. 2019. Oncotarget. 10:993-1009. Tomkowicz B. et al. 2007. Proc Natl Acad Sci U S A. 104:17965-70.
Gene ID: 57124
UniProt: View information about CD248 on UniProt.org
Clone: VI-71
Regulatory Status: RUO
Other Names: TEM1, Endosialin
Isotype: Mouse IgG1, κ