Biolegend, 980424, Spark Violet™ 538 anti-human HLA-DR, 500microl

HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kD α (heavy) chain and a 27 kD β (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells, and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells.
500microl
Reactivity: Human
Formulation: Phosphate-buffered solution, pH 7.2, 0.09% sodium azide, 0.2% (w/v) BSA (origin USA), and a stabilizer.
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Violet™ 538 under optimal conditions.
Concentration: 200 µg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: Suggested for Flow Cytometry
Disclaimer: WARNINGS AND PRECAUTIONS Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet. This antibody contains sodium azide. Follow federal, state and local regulations to dispose of this reagent. Sodium azide build-up in metal wastepipes may lead to explosive conditions; if disposing of reagent down wastepipes, flush with water after disposal. All specimens, samples and any material coming in contact with them should be considered potentially infectious and should be disposed of with proper precautions and in accordance with federal, state and local regulations. Do not use this reagent beyond the expiration date stated on the label. Do not use this reagent if it appears cloudy or if there is any change in the appearance of the reagent as these may be an indication of possible deterioration. Avoid prolonged exposure of the reagent or stained cells to light. Spark Violet™ 538 conjugates may interact with biomolecules present in whole blood samples from certain donors that have been exposed to Polyethylene glycol (PEG) or similar molecules. The interpretation of the staining results must be evaluated by a qualified professional using the appropriate controls.
Antigen References: Levacher M, et al. 1990. Clin Exp Immunol. 81:177-82. Terstappen L, et al. 1990. J Leukoc Biol. 48:138-48. Edwards JA, et al. 1986. J Immunol. 137:490-7. van Es A, et al. 1984. Transplantation. 37:65-9. O'Doherty U, et al. 1994. Immunology. 82:487-93. Thomas R, et al. 1994. J Immunol. 153:4016-28. Grouard G, et al. 1996. Nature. 384:364-7.
Clone: L243
Other Names: Major Histocompatibility Class II, MHC class II
Isotype: Mouse IgG2a, κ