Biolegend, 986806, APC anti-human TNF-α, 500microl

TNF-α is secreted by macrophages, monocytes, neutrophils, T cells, and NK cells. Many transformed cell lines also secrete TNF-α. Monomeric human TNF-α is a 157 amino acid protein (non-glycosylated) with a reported molecular weight of 17 kD. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biological activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.
500microl
Reactivity: Human
Formulation: Phosphate-buffered solution, pH 7.2, 0.09% sodium azide, 0.2% (w/v) BSA (origin USA), and a stabilizer.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 100 µg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: Suggested for Intracellular Staining for Flow Cytometry
Disclaimer: WARNINGS AND PRECAUTIONS Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet. This antibody contains sodium azide. Follow federal, state and local regulations to dispose of this reagent. Sodium azide build-up in metal wastepipes may lead to explosive conditions; if disposing of reagent down wastepipes, flush with water after disposal. All specimens, samples and any material coming in contact with them should be considered potentially infectious and should be disposed of with proper precautions and in accordance with federal, state and local regulations. Do not use this reagent beyond the expiration date stated on the label. Do not use this reagent if it appears cloudy or if there is any change in the appearance of the reagent as these may be an indication of possible deterioration. Avoid prolonged exposure of the reagent or stained cells to light.
Antigen References: Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. Beutler B, et al. 1988. Annu Rev Biochem. 57:505-18. Beutler B, et al. 1989. Annu Rev Immunol. 7:625-55. Tracey K, et al. 1993. Crit Care Med. 21:S415-22.
Clone: MAb11
Other Names: Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2
Isotype: Mouse IgG1, κ