Cobetter, CF-04-F8475, Filter Paper for Western Blot, 0.38 mm thick, Pre-cut Blotting Paper

Check this product with a package size of 7.5 x 8.4 cm / 50/pk on Iright. Western Blotting filter papers are made of high-quality cotton cellulose fiber without any additives of any kind, features a uniform texture and smooth surface. They are produced with ultrapure water which contains no impurities and minimize background signa. Cobetter offers pre-cut sheets for direct use in doing Southern, Northern, and Western transfers, including wet transfer and semi-dry transfer methods. With a medium thickness of 380 μm and dimensions of 7.5×8.4 cm, it is compatible with standard transfer apparatuses and easy to operate. These western blotting filter paper facilitates efficient and rapid transfer of protein molecules from gel to membrane.
Specifications

Material
Cotton Cellulose
Application
Western Blotting Filter Papter
Wettability
Hydrophilic
Thickness
0.38 mm
Dimensions
7.5 x 8.4 cm
20 x 20 cm
Package
50 pcs/pk
20pcs/pk
Features
Convenient: pre-cut sheets to save time
Standard size: compatible with most minigel transfer assemblies
Tested:  compatible with alcohol or other organic solvents commonly used in protein transfer applications 
Applications
Protein transfer
Blot detection
Adsorption analysis
Membrane Transfer Setup
* Membrane transfer set-up, sandwich the filter papers, gel and membrane together
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Why PVDF Transfer Membranes Are Ideal for Western Blotting Applications Q: How should cellulose filter paper be selected for western blot?
The filter paper size should be slightly larger than the gel and the PVDF transfer membrane.
Cobetter pre-cut cellulose filter papers are designed to match the PVDF transfer membranes, offering convenient handling and an optimal fit.
Q: What should be considered when performing Western blotting for a high–molecular-weight protein (~200 kDa)?
Use a lower-percentage resolving gel, preferably around 7%.
Handle the gel carefully during stripping/removal to avoid damage.
Extend the transfer time to ensure efficient protein transfer.
Include a molecular weight marker, otherwise nonspecific bands may be difficult to interpret.
Q: How should transfer conditions be set for Western blotting?
For proteins in the 15–100 kDa range, a typical condition is 300 mA constant current for 45 minutes.
For proteins >100 kDa, lower current (around 220 mA) is generally recommended with a longer transfer time.
Based on experience: ZO-1 (250 kDa) can be successfully transferred in 2.5 hours. 150 kDa proteins can be transferred in 1.5 hours.
For example: If the largest target protein is 200 kDa, the transfer condition can be estimated as: 220 mA × (200 / 150) ≈ 134 minutes, plus an additional ~15–20 minutes, resulting in a total transfer time of approximately 150 minutes.
Q: How can the background on the PVDF western blot membrane be reduced?
1. Ensure proper blocking. PVDF membranes require more stringent blocking steps. This can be achieved by increasing the concentration of the blocking agent 2-5 fold, increasing the blocking time, nd performing the procedure at 37 degrees C.
2. Optimizing the dilution ratios of the primary and secondary antibodies can reduce nonspecific background.
3. Perform thorough washing, and keep the membrane continuously wet and free of contamination during all steps.