Cobetter, HFEMNM0651030P, Biophsep Mini Hollow Fiber Filter mPES Membrane, Microfiltration, 1/pk

Check this product with a package size of 1.0mm / 30cm - EFA: 28 cm² / 0.65 μm on Iright. By offering various lengths, membrane filtration areas, and module diameters, Cobetter hollow fiber modules can facilitate a smooth and predictable scale-up from laboratory to pilot-scale and eventually manufacturing-scale. Available from Mini, Minilab, Lab, Pilot, Pilot+, Process and Max.
Cobetter hollow fiber modules are made of modified polyethersulfone membranes(m-PES), which have high flow flux and low protein binding. They are easy to install, clean and sanitize, which are widely used in cell harvest, clarification, perfusion, diafiltration and concentration in upstream and downstream bioprocessing.
Compare with TFF Cassettes' tortuous and complicated flow path, hollow fiber filtration has a linear flow path which creates a smooth flow for more gentle separation.
Specifications

Materials
Feed/Retentate connectors
Female Luer Lock

Permeate connector
Female Luer lock

Membrane material
mPES
Housing
Polysulfone
End Cap
White-Polysulfone
Seals (internal)
Epoxy Resin
Physical Properties
Microfiltration Pore Size
0.1/0.2/0.45/0.65/0.2-R μm
Fiber ID
1.0 mm
No. Of Fibers
3

Effective Length

30cm
60cm

OD*L (mm*mm)

10*346
10*646

Effective Area

 28cm²
 56cm²

Process Volume

<140mL
<280mL

Flow Rate@2000s-1 (mL/min)
35.3

Flow Rate@6000s-1 (mL/min)
106
Sterility
Delivery Condition
Nonsterile
Package
Pack Size
1 pc/pk
Packaging
Individually packed in PE bag
Features
High flux rates, high filtration capacity
Modified hydrophilic PES hollow fiber membrane provides low protein binding, less membrane fouling and easy cleaning
Integrated device without additional assembly or device holder, quick installation and operation
Re-washed 0.5M NaOH solution
Simple and reliable linear amplified scale-up
Applications
Purification, concentration and diafiltration of vaccine
Purification, concentration and diafiltration of viral vector
Clarification of cells and bacterial in fermentation broth>
Recycling and washing of cells and bacteria
Concentration and diafiltration of protein
Choose Fiber ID
Module with fiber of 0.5mm ID is widely used in most application scenarios to improve mass transfer efficiency.
Module with fiber of 1.0mm ID is ideal for products with high cell density or high solid content or high viscosity.
Only module with fiber of 1.0mm ID is autoclavable, as steam cannot enter the fiber of 0.5mm ID effectively.
Share Rate
The circulating flow rate of the hollow fiber depends on the product tolerance to the shear rate, in generally it will be set at 2000/s to 10000/s, which is much smaller than the shear rate generated by the turbulent flow on the surface of the cassette screen.
For general materials, we usually choose a shear rate of 4000/s to 6000/s;
If the product is sensitive to shear force (such as lentivirus, coronavirus or macromolecular protein expressed by animal cells, large plasmids and LNP, etc.), the shear rate needs to be reduced to 2000/s;
If the product is with good resistance to shear force (such as small molecular proteins expressed by bacteria, etc.) shear rate could be increased to 8000/s to 10000/s.
How to choose hollow fiber MWCO?
Sufficient separation efficiency and the risks of fouling should both be taken into consideration when choosing MWCO.
To minimize the risk of fouling that inevitably occurs during membrane processing, it is advisable to choose membranes with relatively smaller pore sizes, as long as the separation efficiency and flux are maintained. This helps reduce the intrusion of impurity particles into the membrane pores, thus extending the membrane's service life.
Common processing scenarios are as follows:
Heat source removal of small molecule in Chinese medicines: 5kD, 10kD
Ultrafiltration and concentration of mABs, buffer exchange: 30kD, 50kD
Concentration, purification and removal of virus: 100kD, 300kD, 500kD, 750kD
Clarification of recombinant protein, antibody: 500kD, 750kD
Concentration for bacteria: 500kD, 750kD
Clarification of lysate: 0.2μm, 0.45μm, 0.65μm
Effective Length and Scale-Up
The process scale-up feature of hollow fibers is that: direct process scale-up can be carried out as long as the effective length is kept the same.
On the opposite, due to the significant pressure drop difference between inlet and outlet existed in different lengths, the internal pressure and flow velocity distribution of the flow channel also change correspondingly, so linear scale-up can not be performed on devices with different length. When processing with more fouling and high viscosity product, it is preferred to choose module with shorter length.
How to use a hollow fiber membrane filter?
Module Preparation
For lab and pilot applications, you will need a pump, a feed tank, a permeate collection tank, pressure gauges and valves and suitable tubing and connectors.Cobetter hollow fiber modules made with hydrophilic modified PES membrane contain glycerin to preserve the pore structure. Glycerine is water soluble and easy to rinse out.
Rinse out glycerine with DI water with an inlet pressure of 2-5 psi, the flushing volume should be greater than 20L/m².
Drain module and perform module integrity test.
 Note: If sanitization is necessary, use 0.1N NaOH solution as a disinfectant.
 
Module Integrity Test
All hollow fiber modules are integrity tested at our site. However, Cobetter highly recommends an integrity test before use. Cobetter recommends two test methods, one of which may be applied according to actual operating conditions.  
Diffusion Flow Method
Pressure Retaining Method
 
Processing Buffer Equilibrium
The purpose of flushing hollow fiber modules with buffer is to ensure that the pH and salt ion conditions of the system are as similar as possible to the initial state of the sample, so as to reduce pollution and product loss.
 
Cleaning after Use
The hollow fiber modules must be cleaned before storage and reused by flushing with buffer or DI water, and then washed with appropriate sanitizing agent. 0.5M NaOH solution may be used to circulate for 30 min at 20-40°C.
 
Hollow Fiber Storage
The hollow fiber modules may be stored in 0.1N NaOH solution. For long-term storage between 1-12 months, it is recommended to be stored under refrigeration at 4. Q: What are the advantages of hollow fiber compared to cassette (flat-sheet) modules?
1. More open flow channels and relatively lower shear.
2. High packing density and large surface area per module enable a compact system footprint with minimal hold-up volume.
3. Robust handling of challenging feed streams, including high solids content or viscosity.
4. Suited for large-volume, high-flow continuous processing in drug manufacturing, such as for insulin and animal vaccines.
Q: What are the applications of hollow fiber technology?
Concentration and buffer exchange of viral vaccines.
Concentration and diafiltration of Cell and Gene Therapy (CGT) products.
Purification, concentration, and buffer exchange of mRNA products.
Biomass harvesting from bacterial fermentation, and clarification/removal of cell debris from cell culture lysates.
Cell retention in perfusion culture (ATF system).
Q: What materials and cut-offs are available for Cobetter ultrafiltration hollow fiber?
Material: m-PES.
Ultrafiltration HF are available cut-offs ranging from 3–750 kDa.
Microfiltration HF are available pore sizes ranging from 0.1–0.65 µm.
Q: Can Cobetter‘s hollow fiber modules be sterilized?
Specific series are compatible with sterilization procedures, for example, 50 kDa, 750 kDa, and 0.2 μm modules.