Cobetter, HFEMP01000560SP, Biophsep MiniProcess Hollow Fiber Filter Set with Bag Ultrafiltration, Gamma Sterilized 1/pk

Check this product with a package size of 0.5mm / 60cm - EFA: 1.37 m² / 100 kDa on Iright. Designed for single use, eliminating the need for post-use ClP cleaning, regeneration, and cleaning validation. This significantly saves operational time and avoids the risk of cross-contamination.
Pre-treated to reduce TOC and conductivity levels, effectively minimizing endotoxin and bioburden. No need for pre-use alkaline washing to remove pyrogens, enabling rapid experimental setup. Each filter is individually packaged and gamma-irradiated before shipment to ensure sterility.
Specifications

Materials
Feed/Retentate connectors
1.5" TC (compatible with 1-1/2" TC fitting)
Permeate connector
0.5" TC (compatible with 3/4" TC fitting)
Membrane material
mPES
Housing
Polysulfone
End Cap
White-Polysulfone
Seals (internal)
Epoxy Resin
Physical Properties
Fiber ID

0.5mm
1.0mm

No. of Fibers

1,450
600

Effective Length

30cm60cm110cm
30cm60cm110cm

OD*L (mm*mm)
30cm: 47*40060cm: 47*700110cm: 47*1,150
Effective Area

30cm: 0.68 m²60cm: 1.37 m²110cm: 2.50 m²
30cm: 0.56 m²60cm: 1.13 m²110cm: 2.07 m²

Process Volume

30cm: <75 L60cm: <150 L110cm: <250 L
30cm: <75 L60cm: <150 L110cm: <250 L

Flow Rate@2000s-1 (mL/min)

2,132
7,059

Flow Rate@6000s-1 (mL/min)

6,397
21,176

Sterility
Delivery Condition
Sterile (Single use)
Sterilization Method
Gamma Irradiation at 25-45 kGy
Package
Pack Size
1 pc/pk
Packaging
Individually packed in PE bag

Features
Pre-sterilized with gamma irradiation at an intensity of 25-45 kGy
Pre-treated to reduce conductivity and TOC residue, minimizing wetting and shortening installation time
Single-use design eliminates the need for post-use ClP cleaning
Applications
Yeast, oncolytic bacteria, and other cell harvesting/purification
Continuous cell perfusion culture
Aseptic concentration of plasmids and mRNA in CGT applications
Aseptic purification of recombinant proteins
Hollow Fiber Filter Configurations Compared
Sterilization Compatibility Comparison
Choose Fiber ID
Module with fiber of 0.5mm ID is widely used in most application scenarios to improve mass transfer efficiency.
Module with fiber of 1.0mm ID is ideal for products with high cell density or high solid content or high viscosity.
Only module with fiber of 1.0mm ID is autoclavable, as steam cannot enter the fiber of 0.5mm ID effectively.
Share Rate
The circulating flow rate of the hollow fiber depends on the product tolerance to the shear rate, in generally it will be set at 2000/s to 10000/s, which is much smaller than the shear rate generated by the turbulent flow on the surface of the cassette screen.
For general materials, we usually choose a shear rate of 4000/s to 6000/s;
If the product is sensitive to shear force (such as lentivirus, coronavirus or macromolecular protein expressed by animal cells, large plasmids and LNP, etc.), the shear rate needs to be reduced to 2000/s;
If the product is with good resistance to shear force (such as small molecular proteins expressed by bacteria, etc.) shear rate could be increased to 8000/s to 10000/s.
How to choose hollow fiber MWCO?
Sufficient separation efficiency and the risks of fouling should both be taken into consideration when choosing MWCO.
To minimize the risk of fouling that inevitably occurs during membrane processing, it is advisable to choose membranes with relatively smaller pore sizes, as long as the separation efficiency and flux are maintained. This helps reduce the intrusion of impurity particles into the membrane pores, thus extending the membrane's service life.
Common processing scenarios are as follows:
Heat source removal of small molecule in Chinese medicines: 5kD, 10kD
Ultrafiltration and concentration of mABs, buffer exchange: 30kD, 50kD
Concentration, purification and removal of virus: 100kD, 300kD, 500kD, 750kD
Clarification of recombinant protein, antibody: 500kD, 750kD
Concentration for bacteria: 500kD, 750kD
Clarification of lysate: 0.2μm, 0.45μm, 0.65μm
Effective Length and Scale-Up
The process scale-up feature of hollow fibers is that: direct process scale-up can be carried out as long as the effective length is kept the same.
On the opposite, due to the significant pressure drop difference between inlet and outlet existed in different lengths, the internal pressure and flow velocity distribution of the flow channel also change correspondingly, so linear scale-up can not be performed on devices with different length. When processing with more fouling and high viscosity product, it is preferred to choose module with shorter length. Q: What are the advantages of hollow fiber compared to cassette (flat-sheet) modules?
1. More open flow channels and relatively lower shear.
2. High packing density and large surface area per module enable a compact system footprint with minimal hold-up volume.
3. Robust handling of challenging feed streams, including high solids content or viscosity.
4. Suited for large-volume, high-flow continuous processing in drug manufacturing, such as for insulin and animal vaccines.
Q: What are the applications of hollow fiber technology?
Concentration and buffer exchange of viral vaccines.
Concentration and diafiltration of Cell and Gene Therapy (CGT) products.
Purification, concentration, and buffer exchange of mRNA products.
Biomass harvesting from bacterial fermentation, and clarification/removal of cell debris from cell culture lysates.
Cell retention in perfusion culture (ATF system).
Q: What materials and cut-offs are available for Cobetter ultrafiltration hollow fiber?
Material: m-PES.
Ultrafiltration HF are available cut-offs ranging from 3–750 kDa.
Microfiltration HF are available pore sizes ranging from 0.1–0.65 µm.
Q: Can Cobetter‘s hollow fiber modules be sterilized?
Specific series are compatible with sterilization procedures, for example, 50 kDa, 750 kDa, and 0.2 μm modules.