Cobetter, ULRC0020150P-4, Briscale 15mL Ultrafiltration Centrifugal Filter with Regenerated Cellulose RC Membrane

Check this product with a package size of 2 kDa / 7.4cm² / 4/pk on Iright. Cobetter BriScale 15mL centrifugal filters are made of low-binding regenerated cellulose(RC) ultrafiltration membranes, which can achieve a high protein recovery rate >90%, using marker protein Lysozyme(14.4 kDa) at 0.25 mg/mL, centrifuge 60 mins. BriScale centrifugal filter is ideal for the concentration of proteins and biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms. The vertical design and membrane surface area provide fast concentration time and rapid protein concentration for up to 80-fold concentration. The centrifugal filter unit consists of a lid, a filter device and a centrifuge tube. Use a pipette to collect the concentrate from the filter device. The ultrafiltrate is collected in the centrifuge tube for further analysis. BriScale 15 RC Centrifugal Filters can handle samples up to 15 mL in rotors accepting 50 mL centrifuge tubes. Use in fixed angle 45°rotor(max starting volume of 12mL) or swing bucket rotor(max starting volume of 15mL). Specifications Dimensions Effective membrane area 7.4cm² Overall Length x Tube diameter 119.6 x 29 mm Materials Membrane Material Regenerated Cellulose (RC) Centrifuge Tube PP Ultrafiltration device Polystyrene (GPPS) Lid High Density Polyethylene (HDPE) Concentrator Capacity Fixed Angle Rotor(45°) Swing Bucket Rotor Maximum Sample Volume 12 mL 15 mL Dead-stop volume 60 μL 100 μL Final concentration volume 150-300μL Centrifuge Fixed Angle Rotor(45°) Swing Bucket Rotor Maximum G-force 5000g 4000g Sterility Delivery Condition Nonsterile Sterilization Method Not Recommended Package Pack Size 4pcs or 24pcs Packaging Individually packed in PE bag Features RC membrane achieves a high recovery rate of>90% Color-coded cap with different MWCO, volume graduation for easy detection and user-friendly Low-binding regenerated cellulose(RC) ultrafiltration membranes Broad chemical compatibility pH 1-14 80-fold concentration with vertical membranes Translucent housing and volume marker make sample monitoring effortless Applications Concentration and purification of nucleic acids, proteins such as antigens, antibodies, enzymes, etc and other biomolecules Desalting, buffer exchange and dialyzing proteins and antibodies for sample preparation Concentration of dilute or pre-purified proteins from chromatography Remove primers, adapters or molecular tags from the reaction mixture Purification of macromolecules in tissue culture extracts or cell lysates, and protein removal prior to HPLC and mass spectrometry How to choose Cobetter Centrifugal Filters? To choose which type to use, determine the molecular weight (MW) of your macromolecule of interest, and select an ultrafiltration membrane with a molecular weight cut-off (MWCO) that is 2-3 times smaller than the MW of your macromolecule. Read more details about how to choose MWCO filters. Filter MWCO Target MW 3 kDa 6 kDa < MW < 30 kDa 10 kDa 30 kDa < MW < 100 kDa 30 kDa 100 kDa < MW < 200 kDa 50 kDa 200 kDa < MW < 500 kDa 100 kDa 500 kDa < MW Centrifugal Filter Units Structure & Adapted Equipment Cobetter ultrafiltration centrifugal filter units consist of a lid, a filter unit, and a centrifuge tube. It requires a rotor or rotor adapter compatible with 15mL (for 4mL centrifugal filter) or 50mL (for 15mL centrifugal filter) conical bottom centrifuge tubes. Both the swinging bucket rotor and the fixed-angle rotor must meet this requirement. Learn more about how to use a centrifugal filter. Protein Recovery Rate The RC membrane and smooth inner wall design have extremely low protein adsorption. Can achieve a recovery rate of more than 90%. Q: Which centrifuges are Cobetter centrifugal filters compatible with? Briscale UF-15 centrifugal filters are compatible with centrifuges that accept 50 mL conical centrifuge tubes. Briscale UF-4 centrifugal filters are compatible with centrifuges that accept 15 mL conical centrifuge tubes. Both are suitable for horizontal rotors and fixed-angle rotors. Q: How to choose the appropriate centrifugal filters? When selecting a centrifugal filter, the main considerations are the molecular weight of the target molecule and the desired concentration volume. For selecting the membrane cut-off, a general guideline is to choose a cut-off no greater than 1/3 of the target protein’s molecular weight. For example, if the target protein has a molecular weight of 35 kDa, an ultrafiltration tube with a 10 kDa cut-off would be appropriate. Q: How should centrifugal filters be cleaned and stored? Cleaning: Soak the tube in 0.1–0.5 N NaOH solution for 30–60 minutes. Discard the NaOH solution, then centrifuge with ultrapure or deionized water for 10 minutes, repeating 1–2 times. Storage: Fill the tube with 0.05–0.1 N NaOH solution and store immersed. Q: Are Cobetter centrifugal filter single-use? Can they be reused? Cobetter Briscale UF ultrafiltration centrifugal filters are single-use consumables. For the same feed material, they can be reused 6–10 times with 30 min centrifugation per use without issues. For different feed materials, it is recommended to use a new centrifugal filter. Q: Can Cobetter centrifugal filters be sterilized? Cobetter Briscale UF ultrafiltration centrifugal filters are non-sterile and cannot be autoclaved. They can be disinfected using 0.1–0.5 N NaOH or 70% ethanol. Q: What cause low recovery rate after ultrafiltration? There are four possible reasons: 1. Inappropriate ultrafiltration cut-off leading to product loss: Try using a smaller cut-off, generally following the 1/3 rule. For example, for a 10 kDa target molecule, the preferred cut-off is 3 kDa, while also testing 10 kDa and 30 kDa tubes to compare recovery. 2. Excessive concentration factor leading to incomplete recovery: Consider reducing centrifugation time and controlling the sample load. 3. Product integrity failure: Compare two centrifugal filters using buffer or pure water. A damaged centrifugal filter typically shows faster flow and lower concentrated volume. 4. Adsorption effects: If the initial sample concentration is below 0.1 mg/mL, adsorption inside the tube may reduce recovery. Q: Why does protein precipitation occur during concentration? Protein precipitation may occur when the protein is concentrated too rapidly or to an excessively high concentration. Recommended optimization strategies: 1. Reduce the loading volume or centrifugation time to lower the final protein concentration. 2. Decrease the centrifugal force or centrifugation speed. 3. Increase mixing steps, breaking a single long centrifugation into multiple shorter centrifugation cycles.