CST, 10001T, Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying . Validated for Western Blotting,Peptide ELISA (DELFIA). Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit Monoclonal Antibody (CST #10001) is ready to ship. Product Usage Information Western Blotting: 1:1000 Peptide ELISA (DELFIA): 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit Monoclonal Antibody recognizes endogenous proteins containing phospho-Ser/Thr preceded by Arg at positions -5 and -3 in a manner largely independent of the surrounding amino acid sequence. Minor cross-reactivity is observed for proteins that contain phospho-Ser/Thr preceded by Arg at position -3 only. No cross-reactivity is observed with the corresponding nonphosphorylated sequences or with other phospho-Ser/Thr-containing motifs. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with an Akt substrate peptide library. Background An important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt, and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Akt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis, and transcriptional regulation (3-5). While a number of Akt substrates are known (such as GSK-3, Bad, and caspase-9) many important substrates await discovery. Akt phosphorylates substrates only at Ser/Thr in a conserved motif characterized by Arg at positions -5 and -3 (6). Phospho-Akt substrate-specific antibodies from Cell Signaling Technology are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug discovery. Specification REACTIVITY: All SENSITIVITY: Endogenous Source/Isotype: Rabbit