CST, 10197SF, ApoE (pan) (D7I9N) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying ApoE. Validated for WB,IHC,IF,IF,ELISA+. Highly specific and rigorously validated in-house, ApoE (pan) (D7I9N) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #10197) is ready to ship. Product Usage Information This product is the carrier-free version of product #13366. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 13366 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity ApoE (pan) (D7I9N) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total ApoE protein. This antibody also recognizes overexpressed ApoE2, ApoE3, and ApoE4 proteins. Mouse cross-reactivity is determined by ELISA. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu197 of human ApoE protein. Background Apolipoproteins are plasma lipoproteins that function as transporters of lipids and cholesterol in the circulatory system. Chylomicrons are a fundamental class of apolipoproteins containing very low-density lipoproteins (VLDL), intermediate-density lipoproteins (IDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL) (1,2). Alternate Names AD2; Apo-E; APOE; Apolipoprotein E; LDLCQ5; LPG; MGC1571 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 35 Source/Isotype: Rabbit IgG