CST, 10450T, CUL3 (E4G3M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CUL3. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CUL3 (E4G3M) Rabbit Monoclonal Antibody (CST #10450) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CUL3 (E4G3M) Rabbit Monoclonal Antibody recognizes endogenous levels of total CUL3 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala755 of human CUL3 protein. Background CUL3 (Cullin-3) is a member of the cullin-based ubiquitin ligase family. By interacting with Hrt1 and BTB domain containing proteins, the complex functions as a CUL3-based E3 ligase to bring specific substrates to ubiquitinylation and degradation (1). The CUL3 complex has been shown to target many substrates involved in cell cycle progression (2), transcription (3), development and differentiation (4,5). Alternate Names CUL-3; CUL3; cullin 3; Cullin-3; KIAA0617; PHA2E Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 82, 84 Source/Isotype: Rabbit IgG