CST, 10459S, Kindlin-3 (D8I7V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying kindlin-3. Validated for WB,IP,IHC,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Kindlin-3 (D8I7V) Rabbit Monoclonal Antibody (CST #10459) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:400 Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Kindlin-3 (D8I7V) Rabbit Monoclonal Antibody recognizes endogenous levels of total Kindlin-3 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg648 of human Kindlin-3 protein. Background The kindlin family of focal adhesion proteins is involved in multiple biological processes, including integrin signaling, adhesion, migration, angiogenesis, differentiation, and mitotic spindle formation (1,2). Kindlin family members 1, 2, and 3 (FERM1, FERM2, and URP2) are differentially expressed in tissues. Kindlin-1 is primarily expressed in epithelial cells, kindlin-2 is ubiquitously expressed, and kindlin-3 expression is restricted to the hematopoietic system (3). Kindlin-3 (FERMT3 or URP2) is a member of the kindlin family of focal adhesion proteins, which plays an essential role in integrin activation and in various immune functions (3,4). Kindlin-3 is mainly expressed in the hematopoietic system (5). Kindlin-3, together with talin, binds to the cytoplasmic tail of β-integrin and activates integrin-mediated signaling cascades (6-8). Research studies identified mutations of Kindlin-3 that cause a rare hereditary human disease, leukocyte-adhesion deficiency (LAD) type III (9,10). Alternate Names Fermitin family homolog 3; fermitin family homolog 3 (Drosophila); fermitin family member 3; FERMT3; KIND3; kindlin 3; Kindlin-3; MGC10966; MIG-2; MIG2-like protein; MIG2B; UNC-112 related protein 2; Unc-112-related protein 2; UNC112C; URP2; URP2SF Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 75 Source/Isotype: Rabbit IgG