CST, 10473S, NSC-697923

Chemical Modulators for studying in the research area. Product Usage Information NSC-697923 is supplied as a lyophilized powder. For a 10 mM stock, reconstitute 1 mg of powder in 0.37 mL of DMSO. Working concentrations and length of treatment can vary depending on the desired effect. Storage Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles . Background NSC-697923 is a small-molecule inhibitor of the E2 ubiquitin-conjugating enzyme Ubc13-Uev1A. NSC-697923 blocked formation of Ubc13 and ubiquitin thioester conjugates, and suppressed constitutive NF-κB activity in diffuse large B cell lymphoma cells. Inhibition of ubiquitin-dependent DNA damage signaling was observed, but not formation of the phosphorylated form of histone variant H2AX, which is consistent with NSC-697923 targeting of nuclear Ubc13 (1). Additional data suggested that NSC-697923 interacted with the Ubc13 active site cysteine (2). UBE2N (Ubc13) is an E2 ubiquitin-conjugating enzyme that catalyzes K63-linked poly-Ub chain formation. UBE2N forms a heterodimer with MMS2 or Uev1A to exert its E2 ligase function (3). NSC-697923 treatment of neuroblastoma cells increased apoptosis and nuclear p53 accumulation, and enhanced tumor suppression (4). NSC-697923 also inhibited caspase-1 enzymatic activity (IC = 1.737 μM) and NLRP3 protein expression by suppressing the NF-κB signaling pathway. NSC-697923 inhibited joint swelling and NLRP3 inflammasome activation in an animal model of gouty arthritis (5). Alternate Names 2-[(4-Methylphenyl)sulfonyl]-5-nitrofuran